Follicular Fluid Soluble Receptor for Advanced Glycation End-Products (sRAGE): A Potential Indicator of Ovarian Reserve

Follicular Fluid Soluble Receptor for Advanced Glycation End-Products (sRAGE): A Potential Indicator of Ovarian Reserve

Context: The interaction of advanced glycation end-products (AGEs) with their cellular receptor (RAGE) is implicated in the pathogenesis of abnormal ovarian follicular growth. RAGE has a circulating secretory receptor form, soluble RAGE (sRAGE), which neutralizes the action of AGEs and might exert a...

Full description

Saved in:
Bibliographic Details
Published in:The journal of clinical endocrinology and metabolism Vol. 99; no. 2; pp. E226 - E233
Main Authors: Merhi, Zaher, Irani, Mohamad, Doswell, Angela D, Ambroggio, Jennifer
Format: Journal Article
Language:English
Published: United States Endocrine Society 01-02-2014
Copyright by The Endocrine Society
Subjects:
Adult
Age Factors
Anti-Mullerian Hormone - blood
Biomarkers - metabolism
Body Mass Index
Female
Follicle Stimulating Hormone - blood
Follicular Fluid - metabolism
Granulosa Cells - metabolism
Humans
Oocyte Retrieval
Oocytes - cytology
Oocytes - metabolism
Ovarian Follicle - metabolism
Ovulation Induction
Receptor for Advanced Glycation End Products
Receptors, Immunologic - blood
Receptors, Immunologic - genetics
Receptors, Immunologic - metabolism
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:Context: The interaction of advanced glycation end-products (AGEs) with their cellular receptor (RAGE) is implicated in the pathogenesis of abnormal ovarian follicular growth. RAGE has a circulating secretory receptor form, soluble RAGE (sRAGE), which neutralizes the action of AGEs and might exert a protective role on the follicular environment. Objective: The objective of the study was to investigate whether serum or follicular fluid (FF) sRAGE levels are associated with markers of ovarian reserve. Design, Setting, and Participants: Serum anti-Mullerian hormone (AMH) and sRAGE protein levels were correlated in 31 reproductive-aged women. An additional 33 women who underwent oocyte retrieval for in vitro fertilization were enrolled. AMH and its receptor (AMHR-II) mRNA levels were quantified in cumulus granulosa cells and FF sRAGE and AMH protein levels were measured. Main Outcome Measures: Granulosa cell AMH and AMHR-II gene expression, serum and FF AMH and sRAGE protein concentration, and number of oocytes retrieved were measured. Results: In the serum, sRAGE levels were negatively correlated with body mass index (BMI) (r = −0.5, P < .001) but not with age or serum AMH. The higher the FF sRAGE, the lower the number of international units of gonadotropin needed per cycle independent of age, BMI, or day 3 FSH level (r = −0.4, P = .04). After adjusting for age, BMI, day 3 FSH, and the total dose of gonadotropins, FF sRAGE predicted the number of oocytes retrieved (R2 = 0.27, P = .045). FF sRAGE positively correlated with FF AMH levels (r = 0.5, P = .0085). RT-PCR results showed no correlation between the FF sRAGE and AMH or AMHR-II mRNA levels. Conclusion: These data support a relationship between FF sRAGE and measures of ovarian reserve. The pathological significance of the harmful inflammatory AGEs in follicular health clearly requires further investigation. Targeting AGEs might offer potential therapeutic options for the treatment of diminished ovarian response.
Bibliography:This work was supported by grants from the University of Vermont College of Medicine Internal Funds and the American Society for Reproductive Medicine (ASRM).
ObjectType-Article-1
SourceType-Scholarly Journals-1
ObjectType-Feature-2
content type line 23
ISSN:0021-972X
1945-7197
DOI:10.1210/jc.2013-3839