The genes for secretion and maturation of lactococcins are located on the chromosome of Lactococcus lactis IL1403

Southern hybridization and PCR analysis were used to show that Lactococcus lactis IL1403, a plasmid-free strain that does not produce bacteriocin, contains genes on its chromosome that are highly homologous to lcnC and lcnD and encode the lactococcin secretion and maturation system. The lcnC and lcn...

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Bibliographic Details
Published in:	Applied and Environmental Microbiology Vol. 62; no. 5; pp. 1689 - 1692
Main Authors:	Venema, K. (North Carolina State University, Raleigh, NC.), Dost, M.H.R, Beum, P.A.H, Haandrikman, A.J, Venema, G, Kok, J
Format:	Journal Article
Language:	English
Published:	Washington, DC American Society for Microbiology 01-05-1996
Subjects:	Amino Acid Sequence Bacteria BACTERIOCINAS BACTERIOCINE Bacteriocins - genetics Base Sequence Biological and medical sciences Biology of microorganisms of confirmed or potential industrial interest Biotechnology Cellular biology CHROMOSOME Chromosome Mapping Cloning, Molecular COMPOSICION QUIMICA COMPOSITION CHIMIQUE CROMOSOMAS DNA, Bacterial - genetics Fundamental and applied biological sciences. Psychology GENE GENES Genetics LACTOCOCCUS LACTIS Lactococcus lactis - genetics Lactococcus lactis - metabolism LOCALISATION DE GENE LOCALIZACION DE GENES Mission oriented research Molecular Sequence Data MUTACION MUTATION PROTEINAS PROTEINE PROTEOLISIS PROTEOLYSE SECRECION SECRETION SECUENCIA NUCLEICA SEQUENCE NUCLEIQUE TECHNIQUE ANALYTIQUE TECNICAS ANALITICAS Genetic mapping Lactococcus lactis Lactic acid bacteria Molecular processing Nucleotide sequence Secretion Bacteriocin Chromosome Homology Streptococcaceae Bacteria Micrococcales Mutation Molecular cloning
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Summary:	Southern hybridization and PCR analysis were used to show that Lactococcus lactis IL1403, a plasmid-free strain that does not produce bacteriocin, contains genes on its chromosome that are highly homologous to lcnC and lcnD and encode the lactococcin secretion and maturation system. The lcnC and lcnD homologs on the chromosome of IL1403 were interrupted independently by Campbell-type integrations. Both insertion mutants were unable to secrete active lactococcin. Part of the chromosomal lcnC gene was cloned and sequenced. Only a few nucleotide substitutions occurred, compared with the plasmid-encoded lcnC gene, and these did not lead to changes in the deduced amino acid sequence. No genes homologous to those for lactococcin A, B, or M could be detected in IL1403, and the strain does not produce bacteriocin activity
Bibliography:	Q02 9616444 ObjectType-Article-2 SourceType-Scholarly Journals-1 ObjectType-Feature-1 content type line 23 ObjectType-Article-1 ObjectType-Feature-2
ISSN:	0099-2240 1098-5336
DOI:	10.1128/aem.62.5.1689-1692.1996