Proteomic profile of vitrified in vitro-produced bovine embryos (Bos taurus indicus)

The proteomic profile of cryopreserved in vitro produced bovine embryos is little known but can provide insights on the successful application of cryo procedures in support of animal breeding. To identify embryonic proteins and biomarkers related to improved cryotolerance of vitrified in vitro produ...

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Bibliographic Details
Published in:	Cryo-Letters Vol. 43; no. 4; p. 206
Main Authors:	Vieira, J I T, Ferreira-Silva, J C, Silva, F A C, Pena, E P N, Fritas, L C, Chaves, M S, Grazia, J G V, Pereira, L C, Dos Santos Fagundes, R H, Junior, T C, Dos Santos Filho, J P, Freitas, V J F, Oliveira, M A L
Format:	Journal Article
Language:	English
Published:	England 01-07-2022
Subjects:	Animals Blastocyst Cattle Cryopreservation - methods Cryopreservation - veterinary Embryo Culture Techniques - methods Embryo Culture Techniques - veterinary Fertilization in Vitro - veterinary Proteomics Tandem Mass Spectrometry Vitrification
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Summary:	The proteomic profile of cryopreserved in vitro produced bovine embryos is little known but can provide insights on the successful application of cryo procedures in support of animal breeding. To identify embryonic proteins and biomarkers related to improved cryotolerance of vitrified in vitro produced bovine embryos. Proteins were isolated from embryo pools (n = 25 embryos per replicate) and analyzed using the nanoLC - MS/MS system. Further, the UniProtKB database (Uniprot -http://www.uniprot.org/) was used for protein identification. Proteins were classified based on their molecular mass, isoelectric point, and enzymatic activity. Post-translational modification predictions and functional gene ontology analysis were performed as well. Finally, a protein-protein interaction network was created to shed light on the embryo interactome. Based on the MS/MS approach, 66 proteins were identified from vitrified Bos taurus embryos. The retrieved proteins were presumably annotated, which allowed a description of the qualitative and functional aspects of the embryo proteome after the vitrification process. These findings allowed us to conclude that in vitro-produced vitrified embryos expressed proteins that underlie biological processes related to reproduction, stress and lipid metabolic process, which are essential to maintain embryo viability. doi.org/10.54680/fr22410110512.
ISSN:	0143-2044
DOI:	10.54680/fr22410110512