Cytotoxic effects of moderate static magnetic field exposure on human periphery blood mononuclear cells are influenced by Val16Ala-MnSOD gene polymorphism

Technological advancement has increasingly exposed humans to magnetic fields (MFs). However, more insights are necessary into the potential toxicity of MF exposure as a result of genetic variations related to oxidative metabolism. Therefore, the following study has assessed an in vitro cytotoxic eff...

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Bibliographic Details
Published in:	Environmental science and pollution research international Vol. 24; no. 5; pp. 5078 - 5088
Main Authors:	Dornelles, Eduardo B., Goncalves, Bayard D., Schott, Karen Lilian, Barbisan, Fernanda, Unfer, Tais C., Glanzner, Werner G., Machado, Alencar K., Cadona, Francine C., Azzolin, Veronica Farina, Montano, Marco Aurélio Echart, Griner, John, da Cruz, Ivana B. M.
Format:	Journal Article
Language:	English
Published:	Berlin/Heidelberg Springer Berlin Heidelberg 01-02-2017 Springer Nature B.V
Subjects:	Apoptosis Aquatic Pollution Atmospheric Protection/Air Quality Control/Air Pollution B-cell lymphoma BAX protein Bcl-2 protein Bcl-x protein Blood Cells, Cultured Cytometry Cytotoxicity Degenerative diseases Earth and Environmental Science Ecotoxicology Environment Environmental Chemistry Environmental Health Environmental science Exposure Flow cytometry Gene expression Gene polymorphism Genetic diversity Genotype Genotypes Humans Leukocytes (mononuclear) Leukocytes, Mononuclear - drug effects Lymphocytes B Lymphoma Magnetic fields Magnetic Fields - adverse effects Magnetic induction Manganese Metabolism MnSOD gene Oxidation-Reduction Oxidative metabolism Oxidative stress Oxidative Stress - drug effects Polymorphism Polymorphism, Genetic Research Article Superoxide dismutase Superoxide Dismutase - genetics Toxicity Waste Water Technology Water Management Water Pollution Control Oxidative stress Genotype SOD2 PBMC Magnetic field
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Summary:	Technological advancement has increasingly exposed humans to magnetic fields (MFs). However, more insights are necessary into the potential toxicity of MF exposure as a result of genetic variations related to oxidative metabolism. Therefore, the following study has assessed an in vitro cytotoxic effect of static magnetic field (SMF) (5 mT) on cells with Val16Ala polymorphism (AA, VA, and VV) in the manganese superoxide dismutase gene. Homozygous Val16Ala-superoxide dismutase 2 (SOD2) genotypes present oxidative imbalance that is associated with risk to several chronic degenerative diseases (VV produces less efficient and AA more efficient SOD2 enzyme). Blood samples from healthy adult subject carriers with different Val16Ala-SOD2 genotypes were obtained and exposed to MF at different times (0, 1, 3, 6 h). The cytotoxic effect as well as oxidative stress was evaluated after incubation of 24 h at 37 °C. In addition, apoptosis induction has been analyzed by flow cytometry as well as Bcl-2-associated X protein (BAX), B-cell lymphoma 2 (BCL-2), and caspases 8 and 3 gene expression. SMF cytotoxic effect has been observed in AA cells at all times of exposure, whereas AV cells presented higher mortality only after 6 h of exposure at SMF. Higher apoptosis induction has been observed in AA cells when compared to VV and AV cells. These results suggest a toxicogenetic SMF effect related to an imbalance in SOD2 activity.
Bibliography:	ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 23
ISSN:	0944-1344 1614-7499
DOI:	10.1007/s11356-016-8176-x