Self-assembled polymeric nanoparticles as new, smart contrast agents for cancer early detection using magnetic resonance imaging

Early cancer detection is a major factor in the reduction of mortality and cancer management cost. Here we developed a smart and targeted micelle-based contrast agent for magnetic resonance imaging (MRI), able to turn on its imaging capability in the presence of acidic cancer tissues. This smart con...

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Bibliographic Details
Published in:	International journal of nanomedicine Vol. 10; no. default; pp. 63 - 76
Main Authors:	Mouffouk, Fouzi, Simão, Teresa, Dornelles, Daniel F, Lopes, André D, Sau, Pablo, Martins, Jorge, Abu-Salah, Khalid M, Alrokayan, Salman A, Rosa da Costa, Ana M, dos Santos, Nuno R
Format:	Journal Article
Language:	English
Published:	New Zealand Dove Medical Press Limited 01-01-2015 Taylor & Francis Ltd Dove Press Dove Medical Press
Subjects:	Animals Bioengineering Breast cancer Cancer cancer detection Cell Line, Tumor Contrast agents Contrast media Contrast Media - chemistry Diagnosis Early Detection of Cancer - methods Fourier transforms Gadolinium - chemistry HEK293 Cells Humans Hydrophobic and Hydrophilic Interactions Magnetic Resonance Imaging MCF-7 Cells Medical imaging Mesenchymal Stromal Cells Methacrylates - chemistry Methods Mice micelle Micelles Molecular weight Nanoparticles Nanoparticles - chemistry Neoplasms - diagnosis NMR Nuclear magnetic resonance Original Research Permeability pH-sensitive Polymers - chemistry Properties Quantum dots self-assembly smart contrast agent Portugal Kuwait micelle pH-sensitive self-assembly cancer detection smart contrast agent
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Summary:	Early cancer detection is a major factor in the reduction of mortality and cancer management cost. Here we developed a smart and targeted micelle-based contrast agent for magnetic resonance imaging (MRI), able to turn on its imaging capability in the presence of acidic cancer tissues. This smart contrast agent consists of pH-sensitive polymeric micelles formed by self-assembly of a diblock copolymer (poly(ethyleneglycol-b-trimethylsilyl methacrylate)), loaded with a gadolinium hydrophobic complex ((t)BuBipyGd) and exploits the acidic pH in cancer tissues. In vitro MRI experiments showed that (t)BuBipyGd-loaded micelles were pH-sensitive, as they turned on their imaging capability only in an acidic microenvironment. The micelle-targeting ability toward cancer cells was enhanced by conjugation with an antibody against the MUC1 protein. The ability of our antibody-decorated micelles to be switched on in acidic microenvironments and to target cancer cells expressing specific antigens, together with its high Gd(III) content and its small size (35-40 nm) reveals their potential use for early cancer detection by MRI.
Bibliography:	ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 23 These authors contributed equally to this work
ISSN:	1178-2013 1176-9114 1178-2013
DOI:	10.2147/IJN.S71190