Search Results - "Doolan, Padraig"

Correlating transcriptional networks to breast cancer survival: a large-scale coexpression analysis by Clarke, Colin, Madden, Stephen F, Doolan, Padraig, Aherne, Sinead T, Joyce, Helena, O'Driscoll, Lorraine, Gallagher, William M, Hennessy, Bryan T, Moriarty, Michael, Crown, John, Kennedy, Susan, Clynes, Martin

“…Weighted gene coexpression network analysis (WGCNA) is a powerful 'guilt-by-association'-based method to extract coexpressed groups of genes from large…”
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An arginase-based system for selection of transfected CHO cells without the use of toxic chemicals by Capella Roca, Berta, Lao, Nga, Barron, Niall, Doolan, Padraig, Clynes, Martin

“…Polyamines have essential roles in cell proliferation, DNA replication, transcription, and translation processes, with intracellular depletion of putrescine,…”
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Altered gene expression in CHO cells following polyamine starvation by Capella Roca, Berta, Doolan, Padraig, Barron, Niall, O’Neill, Fiona, Clynes, Martin

“…Aim To investigate the impact of polyamine deprivation on the transcriptome of CHO cells Results Polyamines play a central but poorly-understood role in cell…”
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MiR-7 triggers cell cycle arrest at the G1/S transition by targeting multiple genes including Skp2 and Psme3 by Sanchez, Noelia, Gallagher, Mark, Lao, Nga, Gallagher, Clair, Clarke, Colin, Doolan, Padraig, Aherne, Sinead, Blanco, Alfonso, Meleady, Paula, Clynes, Martin, Barron, Niall

“…MiR-7 acts as a tumour suppressor in many cancers and abrogates proliferation of CHO cells in culture. In this study we demonstrate that miR-7 targets key…”
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Integrated miRNA, mRNA and protein expression analysis reveals the role of post-transcriptional regulation in controlling CHO cell growth rate by Clarke, Colin, Henry, Michael, Doolan, Padraig, Kelly, Shane, Aherne, Sinead, Sanchez, Noelia, Kelly, Paul, Kinsella, Paula, Breen, Laura, Madden, Stephen F, Zhang, Lin, Leonard, Mark, Clynes, Martin, Meleady, Paula, Barron, Niall

“…To study the role of microRNA (miRNA) in the regulation of Chinese hamster ovary (CHO) cell growth, qPCR, microarray and quantitative LC-MS/MS analysis were…”
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Predicting cell-specific productivity from CHO gene expression by Clarke, Colin, Doolan, Padraig, Barron, Niall, Meleady, Paula, O'Sullivan, Finbarr, Gammell, Patrick, Melville, Mark, Leonard, Mark, Clynes, Martin

“…Improving the rate of recombinant protein production in Chinese hamster ovary (CHO) cells is an important consideration in controlling the cost of…”
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Parallel mRNA, proteomics and miRNA expression analysis in cell line models of the intestine by O'Sullivan, Finbarr, Keenan, Joanne, Aherne, Sinead, O'Neill, Fiona, Clarke, Colin, Henry, Michael, Meleady, Paula, Breen, Laura, Barron, Niall, Clynes, Martin, Horgan, Karina, Doolan, Padraig, Murphy, Richard

“…AIM To identify mi RNA-regulated proteins differentially expressed between Caco2 and HT-29: two principal cell line models of the intestine.METHODS…”
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Microarray and proteomics expression profiling identifies several candidates, including the valosin-containing protein (VCP), involved in regulating high cellular growth rate in production CHO cell lines by Doolan, Padraig, Meleady, Paula, Barron, Niall, Henry, Michael, Gallagher, Ross, Gammell, Patrick, Melville, Mark, Sinacore, Martin, McCarthy, Kevin, Leonard, Mark, Charlebois, Timothy, Clynes, Martin

“…A high rate of cell growth (μ) leading to rapid accumulation of viable biomass is a desirable phenotype during scale up operations and the early stages of…”
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MAGE-D4B is a novel marker of poor prognosis and potential therapeutic target involved in breast cancer tumorigenesis by Germano, Serena, Kennedy, Susan, Rani, Sweta, Gleeson, Grainne, Clynes, Martin, Doolan, Padraig, McDonnell, Susan, Hughes, Linda, Crown, John, O'Driscoll, Lorraine

“…Melanoma‐associated antigen (MAGE) family members are generally described as tumor‐specific antigens. An association between MAGE‐D4B and breast cancer has yet…”
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Sustained productivity in recombinant Chinese hamster ovary (CHO) cell lines: proteome analysis of the molecular basis for a process-related phenotype by Meleady, Paula, Doolan, Padraig, Henry, Michael, Barron, Niall, Keenan, Joanne, O'Sullivan, Finbar, Clarke, Colin, Gammell, Patrick, Melville, Mark W, Leonard, Mark, Clynes, Martin

“…The ability of mammalian cell lines to sustain cell specific productivity (Qp) over the full duration of bioprocess culture is a highly desirable phenotype,…”
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CGCDB: A web-based resource for the investigation of gene coexpression in CHO cell culture by Clarke, Colin, Doolan, Padraig, Barron, Niall, Meleady, Paula, Madden, Stephen F., DiNino, Dana, Leonard, Mark, Clynes, Martin

“…Coexpression analysis is a powerful, widely used methodology for the investigation of underlying patterns in gene expression data. This “guilt‐by‐association”…”
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Investigation of the molecular profile of basal cell carcinoma using whole genome microarrays by O'Driscoll, Lorraine, McMorrow, Jason, Doolan, Padraig, McKiernan, Eadaoin, Mehta, Jai Prakash, Ryan, Eoin, Gammell, Patrick, Joyce, Helena, O'Donovan, Norma, Walsh, Nicholas, Clynes, Martin

“…Skin cancer accounts for 1/3 of all newly diagnosed cancer. Although seldom fatal, basal cell carcinoma (BCC) is associated with severe disfigurement and…”
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Gene expression changes as markers of early lapatinib response in a panel of breast cancer cell lines by O'Neill, Fiona, Madden, Stephen F, Aherne, Sinead T, Clynes, Martin, Crown, John, Doolan, Padraig, O'Connor, Robert

“…Lapatinib, a tyrosine kinase inhibitor of HER2 and EGFR and is approved, in combination with capecitabine, for the treatment of trastuzumab-refractory…”
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Development and characterization of a Chinese hamster ovary cell-specific oligonucleotide microarray by Melville, Mark, Doolan, Padraig, Mounts, William, Barron, Niall, Hann, Louane, Leonard, Mark, Clynes, Martin, Charlebois, Tim

“…The Chinese hamster ovary (CHO) cell line is one of the most widely used mammalian cell lines for biopharmaceutical production. We have developed and…”
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Transcriptional Profiling of Gene Expression Changes in a PACE-Transfected CHO DUKX Cell Line Secreting High Levels of rhBMP-2 by Doolan, Padraig, Melville, Mark, Gammell, Patrick, Sinacore, Martin, Meleady, Paula, McCarthy, Kevin, Francullo, Linda, Leonard, Mark, Charlebois, Timothy, Clynes, Martin

“…Chinese hamster ovary (CHO) cells are widely used in the biopharmaceutical industry for the production of recombinant human proteins including complex…”
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Feasibility and Relevance of Global Expression Profiling of Gene Transcripts in Serum from Breast Cancer Patients Using Whole Genome Microarrays and Quantitative RT-PCR by O'Driscoll, L, Kenny, E, Mehta, J P, Doolan, P, Joyce, H, Gammell, P, Hill, A, O'Daly, B, O'Gorman, D, Clynes, M

“…Background: Previous studies, by ourselves and others, have indicated that gene transcripts are detectable extracellularly. Advancing on this work, in order to…”
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A Microarray Approach to Translational Medicine in Breast Cancer: How Representative are Cell Line Models of Clinical Conditions? by JAI PRAKASH MEHTA, O'DRISCOLL, Lorraine, BARRON, Niall, CLYNES, Martin, DOOLAN, Padraig

“…The aim of this study was to examine whether the degree to which cell lines model corresponding cells in vivo is an important aspect of their value as models…”
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Serum-Free and Protein-Free Media for the Cultivation of Recombinant Chinese Hamster Ovary (CHO) Cell Lines by Capella Roca, Berta, Meiller, Justine, Doolan, Padraig, Clynes, Martin

“…We describe the preparation of two cell culture media formulations for the culture in suspension of Chinese hamster ovary (CHO) cell lines. The first medium,…”
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Investigation and circumvention of transfection inhibition by ferric ammonium citrate in serum‐free media for Chinese hamster ovary cells by Capella Roca, Berta, Lao, Nga T., Clynes, Martin, Doolan, Padraig

“…While reliable transfection methods are essential for Chinese hamster ovary (CHO) cell line engineering, reduced transfection efficiencies have been observed…”
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Large scale microarray profiling and coexpression network analysis of CHO cells identifies transcriptional modules associated with growth and productivity by Clarke, Colin, Doolan, Padraig, Barron, Niall, Meleady, Paula, O'Sullivan, Finbarr, Gammell, Patrick, Melville, Mark, Leonard, Mark, Clynes, Martin

“…Weighted gene coexpression network analysis (WGCNA) was utilised to explore Chinese hamster ovary (CHO) cell transcriptome patterns associated with bioprocess…”
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