Search Results - "Donohoe, S."
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Characterization and engineering of a plastic-degrading aromatic polyesterase
Published in Proceedings of the National Academy of Sciences - PNAS (08-05-2018)“…Poly(ethylene terephthalate) (PET) is one of the most abundantly produced synthetic polymers and is accumulating in the environment at a staggering rate as…”
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Characterization and engineering of a two-enzyme system for plastics depolymerization
Published in Proceedings of the National Academy of Sciences - PNAS (13-10-2020)“…Plastics pollution represents a global environmental crisis. In response, microbes are evolving the capacity to utilize synthetic polymers as carbon and energy…”
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Disruption of Mediator rescues the stunted growth of a lignin-deficient Arabidopsis mutant
Published in Nature (London) (15-05-2014)“…Disruption of lignin biosynthesis has been proposed as a way to improve forage and bioenergy crops, but it can result in stunted growth and developmental…”
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Visualizing lignin coalescence and migration through maize cell walls following thermochemical pretreatment
Published in Biotechnology and bioengineering (01-12-2008)“…Plant cell walls are composed primarily of cellulose, hemicelluloses, lignins, and pectins. Of these components, lignins exhibit unique chemistry and…”
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Manipulation of Guaiacyl and Syringyl Monomer Biosynthesis in an Arabidopsis Cinnamyl Alcohol Dehydrogenase Mutant Results in Atypical Lignin Biosynthesis and Modified Cell Wall Structure
Published in The Plant cell (01-08-2015)“…Modifying lignin composition and structure is a key strategy to increase plant cell wall digestibility for biofuel production. Disruption of the genes encoding…”
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Reassessing the Roles of PIN Proteins and Anticlinal Microtubules during Pavement Cell Morphogenesis
Published in Plant physiology (Bethesda) (01-01-2018)“…The leaf epidermis is a biomechanical shell that influences the size and shape of the organ. Its morphogenesis is a multiscale process in which nanometer-scale…”
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Revealing Nature's Cellulase Diversity: The Digestion Mechanism of Caldicellulosiruptor bescii CelA
Published in Science (American Association for the Advancement of Science) (20-12-2013)“…Most fungi and bacteria degrade plant cell walls by secreting free, complementary enzymes that hydrolyze cellulose; however, some bacteria use large enzymatic…”
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3D Electron Tomography of Pretreated Biomass Informs Atomic Modeling of Cellulose Microfibrils
Published in ACS nano (24-09-2013)“…Fundamental insights into the macromolecular architecture of plant cell walls will elucidate new structure–property relationships and facilitate optimization…”
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The metagenome of an anaerobic microbial community decomposing poplar wood chips
Published in PloS one (21-05-2012)“…This study describes the composition and metabolic potential of a lignocellulosic biomass degrading community that decays poplar wood chips under anaerobic…”
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Biomass Particle Models with Realistic Morphology and Resolved Microstructure for Simulations of Intraparticle Transport Phenomena
Published in Energy & fuels (15-01-2015)“…Biomass exhibits a complex microstructure of directional pores that impact how heat and mass are transferred within biomass particles during conversion…”
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Cis‐Golgi Cisternal Assembly and Biosynthetic Activation Occur Sequentially in Plants and Algae
Published in Traffic (Copenhagen, Denmark) (01-05-2013)“…The cisternal progression/maturation model of Golgi trafficking predicts that cis‐Golgi cisternae are formed de novo on the cis‐side of the Golgi. Here we…”
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The Multi Domain Caldicellulosiruptor bescii CelA Cellulase Excels at the Hydrolysis of Crystalline Cellulose
Published in Scientific reports (29-08-2017)“…The crystalline nature of cellulose microfibrils is one of the key factors influencing biomass recalcitrance which is a key technical and economic barrier to…”
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Real-Time and Post-reaction Microscopic Structural Analysis of Biomass Undergoing Pyrolysis
Published in Energy & fuels (16-07-2009)“…The structural complexity of unprocessed plant tissues used for thermochemical conversion of biomass to fuels and energy impedes heat and mass transfer and may…”
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Comparative material balances around pretreatment technologies for the conversion of switchgrass to soluble sugars
Published in Bioresource technology (01-12-2011)“…► Material balances improve comparisons of different lignocellulosic pretreatment methods. ► Pretreatment pH strongly effects solubilization and degradation of…”
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Alkaline Pretreatment of Corn Stover: Bench-Scale Fractionation and Stream Characterization
Published in ACS sustainable chemistry & engineering (02-06-2014)“…Biomass pretreatment generally aims to increase accessibility to plant cell wall polysaccharides for carbohydrate-active enzymes to produce sugars for…”
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Ferrous and Ferric Ion-Facilitated Dilute Acid Pretreatment of Lignocellulosic Biomass under Anaerobic or Aerobic Conditions: Observations of Fe Valence Interchange and the Role of Fenton Reaction
Published in Molecules (Basel, Switzerland) (20-03-2020)“…Ferrous ion co-catalyst enhancement of dilute-acid (DA) pretreatment of biomass is a promising technology for increasing the release of sugars from…”
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Chemically Induced Conditional Rescue of the Reduced Epidermal Fluorescence8 Mutant of Arabidopsis Reveals Rapid Restoration of Growth and Selective Turnover of Secondary Metabolite Pools
Published in Plant physiology (Bethesda) (01-02-2014)“…The phenylpropanoid pathway is responsible for the biosynthesis of diverse and important secondary metabolites including lignin and flavonoids. The reduced…”
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Identification and characterization of COPIa- and COPIb-type vesicle classes associated with plant and algal Golgi
Published in Proceedings of the National Academy of Sciences - PNAS (02-01-2007)“…Coat protein I (COPI) vesicles arise from Golgi cisternae and mediate the recycling of proteins from the Golgi back to the endoplasmic reticulum (ER) and the…”
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Towards an Understanding of Enhanced Biomass Digestibility by In Planta Expression of a Family 5 Glycoside Hydrolase
Published in Scientific reports (29-06-2017)“…In planta expression of a thermophilic endoglucanase (AcCel5A) reduces recalcitrance by creating voids and other irregularities in cell walls of Arabidopsis…”
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Elucidation of Biomass Pyrolysis Products Using a Laminar Entrained Flow Reactor and Char Particle Imaging
Published in Energy & fuels (20-01-2011)“…We have mapped the formation of tars during white oak thermochemical conversion using a bench scale laminar entrained flow reactor (LEFR). White oak particles…”
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