Metabolomic and transcriptomic exploration of the uric acid-reducing flavonoids biosynthetic pathways in the fruit of Actinidia arguta Sieb. Zucc

Flavonoids from Actinidia arguta Sieb. Zucc. can reduce uric acid in mice. However, the molecular basis of its biosynthesis is still unclear. In this paper, we used a combination of extensively targeted metabolomics and transcriptomics analysis to determine the types and differences of flavonoids in...

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Bibliographic Details
Published in:	Frontiers in plant science Vol. 13; p. 1025317
Main Authors:	Wang, Yubo, Zhang, Minghui, Dong, Kuiling, Yin, Xiaojuan, Hao, Chunhui, Zhang, Wenge, Irfan, Muhammad, Chen, Lijing, Wang, Yong
Format:	Journal Article
Language:	English
Published:	Frontiers Media S.A 27-10-2022
Subjects:	Actinidia arguta Sieb.Zucc biosynthetic pathway flavonoids Plant Science transcriptome widely targeted metabonomics
Online Access:	Get full text
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Summary:	Flavonoids from Actinidia arguta Sieb. Zucc. can reduce uric acid in mice. However, the molecular basis of its biosynthesis is still unclear. In this paper, we used a combination of extensively targeted metabolomics and transcriptomics analysis to determine the types and differences of flavonoids in the fruit ripening period (August to September) of two main cultivated varieties in northern China. The ethanol extract was prepared, and the potential flavonoids of Chrysin (Flavone1), Rutin (Flavone2), and Daidzein (Flavone3) in Actinidia arguta Sieb. Zucc. were separated and purified by HPD600 macroporous adsorption resin and preparative liquid chromatography. The structure was identified by MS-HPLC, and the serum uric acid index of male Kunming mice was determined by an animal model test.125 flavonoids and 50 differentially regulated genes were identified. The contents of UA (uric acid), BUN (urea nitrogen), Cr (creatinine), and GAPDH in mouse serum and mouse liver glycogen decreased or increased in varying degrees. This paper reveals the biosynthetic pathway of uric acid-reducing flavonoids in the fruit of Actinidia arguta Sieb. Zucc. , a major cultivar in northern China, provides valuable information for the development of food and drug homologous functional foods.
Bibliography:	ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 23 Edited by: Claudia Renate Stange, University of Chile, Chile Reviewed by: Francisca Rodrigues, LAQV Network of Chemistry and Technology, Portugal; Kang Wei, Tea Research Institute, Chinese Academy of Agricultural Sciences (CAAS), China This article was submitted to Plant Biotechnology, a section of the journal Frontiers in Plant Science
ISSN:	1664-462X 1664-462X
DOI:	10.3389/fpls.2022.1025317