Genetic Diversity of Local Greek and Bulgarian Grapevine (Vitis Vinifera L.) Varieties

Genetic Diversity of Local Greek and Bulgarian Grapevine (Vitis Vinifera L.) Varieties

The aim of this study was to estimate the genetic diversity of Greek and Bulgarian grapevine varieties with the use of microsatellite markers. The studied samples were collected from various productive vineyards, consisting of eight Greek and nine Bulgarian native varieties. In order to create a gen...

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Bibliographic Details
Published in:Diversity (Basel) Vol. 12; no. 7; p. 273
Main Authors: Papapetrou, Maria, Loukovitis, Dimitrios, Papadopoulos, Orestis, Kazlari, Zoi, Peristeraki, Anastasia, Arsenova, Slavina, Bardarova, Desislava, Doncheva, Desislava, Theocharis, Serafeim, Karagiannidis, Constantinos, Koundouras, Stefanos, Giannakoula, Anastasia, Aggelopoulos, Stamatis, Chatziplis, Dimitrios
Format: Journal Article
Language:English
Published: Basel MDPI AG 01-07-2020
Subjects:
Alleles
Annotations
Cultivars
Deoxyribonucleic acid
differentiation
DNA
Genetic diversity
Genetic markers
Genetic relationship
Germplasm
grapevine
Grapevines
Heterogeneity
Markers
microsatellite markers
Microsatellites
Polymorphism
Population
Software
Statistical analysis
Statistical methods
Synonymy
Vineyards
Vitis vinifera
Greece
Bulgaria
United States > US
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Summary:The aim of this study was to estimate the genetic diversity of Greek and Bulgarian grapevine varieties with the use of microsatellite markers. The studied samples were collected from various productive vineyards, consisting of eight Greek and nine Bulgarian native varieties. In order to create a genetic profile for each sample, a multiplex PCR reaction method was used amplifying simultaneously seven microsatellite loci. Statistical analysis of data showed that there was a high degree of genetic heterogeneity among most of the varieties studied, highlighting the discriminative power of the chosen set of markers. Moreover, the synonymy of (I) Greek Pamid and Bulgarian Pamid and (II) Greek Zoumiatiko and Bulgarian Dimyat was suggested, as each variety pair had identical allele profiles in all loci examined. Regarding the Greek Mavrud and Bulgarian Mavrud varieties, there was a close genetic relationship between them, however, they did not share common alleles in all microsatellite loci and, therefore, should not be characterized as synonyms. On the other hand, Greek and Bulgarian Keratsouda, which were supposed to be common varieties, were found to be genetically different, supporting that these two varieties should be considered as homonyms. Despite the genotypic assay performed herein, we believe that additional molecular work is needed for the efficient management of Greek and Bulgarian grapevine genepools, as well as to safely suggest any synonym or homonym annotation.
ISSN:1424-2818
1424-2818
DOI:10.3390/d12070273