Search Results - "Donald G. Moerman"

Sarcomeres Pattern Proprioceptive Sensory Dendritic Endings through UNC-52/Perlecan in C. elegans by Liang, Xing, Dong, Xintong, Moerman, Donald G., Shen, Kang, Wang, Xiangming

“…Sensory dendrites innervate peripheral tissues through cell-cell interactions that are poorly understood. The proprioceptive neuron PVD in C. elegans extends…”
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The million mutation project: a new approach to genetics in Caenorhabditis elegans by Thompson, Owen, Edgley, Mark, Strasbourger, Pnina, Flibotte, Stephane, Ewing, Brent, Adair, Ryan, Au, Vinci, Chaudhry, Iasha, Fernando, Lisa, Hutter, Harald, Kieffer, Armelle, Lau, Joanne, Lee, Norris, Miller, Angela, Raymant, Greta, Shen, Bin, Shendure, Jay, Taylor, Jon, Turner, Emily H, Hillier, LaDeana W, Moerman, Donald G, Waterston, Robert H

“…We have created a library of 2007 mutagenized Caenorhabditis elegans strains, each sequenced to a target depth of 15-fold coverage, to provide the research…”
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CRISPR/Cas9 Methodology for the Generation of Knockout Deletions in Caenorhabditis elegans by Au, Vinci, Li-Leger, Erica, Raymant, Greta, Flibotte, Stephane, Chen, George, Martin, Kiana, Fernando, Lisa, Doell, Claudia, Rosell, Federico I, Wang, Su, Edgley, Mark L, Rougvie, Ann E, Hutter, Harald, Moerman, Donald G

“…The Gene Knockout Consortium is tasked with obtaining null mutations in each of the more than 20,000 open reading frames (ORFs) of this organism. To date,…”
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Peel-1 negative selection promotes screening-free CRISPR-Cas9 genome editing in Caenorhabditis elegans by McDiarmid, Troy A, Au, Vinci, Moerman, Donald G, Rankin, Catharine H

“…Improved genome engineering methods that enable automation of large and precise edits are essential for systematic investigations of genome function. We…”
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CRISPR-Cas9 human gene replacement and phenomic characterization in Caenorhabditis elegans to understand the functional conservation of human genes and decipher variants of uncertain significance by McDiarmid, Troy A, Au, Vinci, Loewen, Aaron D, Liang, Joseph, Mizumoto, Kota, Moerman, Donald G, Rankin, Catharine H

“…Our ability to sequence genomes has vastly surpassed our ability to interpret the genetic variation we discover. This presents a major challenge in the…”
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Identification of essential genes in Caenorhabditis elegans through whole-genome sequencing of legacy mutant collections by Li-Leger, Erica, Feichtinger, Richard, Flibotte, Stephane, Holzkamp, Heinke, Schnabel, Ralf, Moerman, Donald G

“…Abstract It has been estimated that 15%–30% of the ∼20,000 genes in C. elegans are essential, yet many of these genes remain to be identified or characterized…”
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Genome-scale analysis of in vivo spatiotemporal promoter activity in Caenorhabditis elegans by Shingles, Jane, Tasan, Murat, Reece-Hoyes, John, Venkatesan, Kavitha, Tu, Domena, Roth, Frederick P, Hidalgo, César A, Bertin, Nicolas, Blanc, Aurélie, Dupuy, Denis, Hunt-Newbury, Rebecca, Rosenberg, Jennifer, Moerman, Donald G, Carnec, Alain, Lee, David, Baillie, David, Johnsen, Robert, Mohler, William A, Vidal, Marc, Barabási, Albert-László, Pulak, Rock, Hope, Ian A, Carvunis, Anne-Ruxandra, Viveiros, Ryan, Le Peuch, Christian, Svrzikapa, Nenad

“…Differential regulation of gene expression is essential for cell fate specification in metazoans. Characterizing the transcriptional activity of gene…”
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High-throughput in vivo analysis of gene expression in Caenorhabditis elegans by Hunt-Newbury, Rebecca, Viveiros, Ryan, Johnsen, Robert, Mah, Allan, Anastas, Dina, Fang, Lily, Halfnight, Erin, Lee, David, Lin, John, Lorch, Adam, McKay, Sheldon, Okada, H Mark, Pan, Jie, Schulz, Ana K, Tu, Domena, Wong, Kim, Zhao, Z, Alexeyenko, Andrey, Burglin, Thomas, Sonnhammer, Eric, Schnabel, Ralf, Jones, Steven J, Marra, Marco A, Baillie, David L, Moerman, Donald G

“…Using DNA sequences 5' to open reading frames, we have constructed green fluorescent protein (GFP) fusions and generated spatial and temporal tissue expression…”
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Establishment of a tissue-specific RNAi system in C. elegans by Qadota, Hiroshi, Inoue, Makiko, Hikita, Takao, Köppen, Mathias, Hardin, Jeffrey D., Amano, Mutsuki, Moerman, Donald G., Kaibuchi, Kozo

“…In C. elegans, mosaic analysis is a powerful genetic tool for determining in which tissue or specific cells a gene of interest is required. For traditional…”
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Identification of genes expressed in the hermaphrodite germ line of C. elegans using SAGE by Wang, Xin, Zhao, Yongjun, Wong, Kim, Ehlers, Peter, Kohara, Yuji, Jones, Steven J, Marra, Marco A, Holt, Robert A, Moerman, Donald G, Hansen, Dave

“…Germ cells must progress through elaborate developmental stages from an undifferentiated germ cell to a fully differentiated gamete. Some of these stages…”
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PhenoMIP: High-Throughput Phenotyping of Diverse Caenorhabditis elegans Populations via Molecular Inversion Probes by Mok, Calvin, Belmarez, Gabriella, Edgley, Mark L, Moerman, Donald G, Waterston, Robert H

“…Abstract Whether generated within a lab setting or isolated from the wild, variant alleles continue to be an important resource for decoding gene function in…”
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A Molecular Mechanism for the Requirement of PAT-4 (Integrin-linked Kinase (ILK)) for the Localization of UNC-112 (Kindlin) to Integrin Adhesion Sites by Qadota, Hiroshi, Moerman, Donald G., Benian, Guy M.

“…Caenorhabditis elegans muscle cells attach to basement membrane through adhesion plaques. PAT-3 (β-integrin), UNC-112 (kindlin), and PAT-4 (integrin-linked…”
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The molecular signature and cis-regulatory architecture of a C. elegans gustatory neuron by Etchberger, John F, Lorch, Adam, Sleumer, Monica C, Zapf, Richard, Jones, Steven J, Marra, Marco A, Holt, Robert A, Moerman, Donald G, Hobert, Oliver

“…Taste receptor cells constitute a highly specialized cell type that perceives and conveys specific sensory information to the brain. The detailed molecular…”
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Accelerating Gene Discovery by Phenotyping Whole-Genome Sequenced Multi-mutation Strains and Using the Sequence Kernel Association Test (SKAT) by Timbers, Tiffany A, Garland, Stephanie J, Mohan, Swetha, Flibotte, Stephane, Edgley, Mark, Muncaster, Quintin, Au, Vinci, Li-Leger, Erica, Rosell, Federico I, Cai, Jerry, Rademakers, Suzanne, Jansen, Gert, Moerman, Donald G, Leroux, Michel R

“…Forward genetic screens represent powerful, unbiased approaches to uncover novel components in any biological process. Such screens suffer from a major…”
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Copy number variation in the genomes of twelve natural isolates of Caenorhabditis elegans by Maydan, Jason S, Lorch, Adam, Edgley, Mark L, Flibotte, Stephane, Moerman, Donald G

“…Copy number variation is an important component of genetic variation in higher eukaryotes. The extent of natural copy number variation in C. elegans is unknown…”
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ELT-2 is the predominant transcription factor controlling differentiation and function of the C. elegans intestine, from embryo to adult by McGhee, James D., Fukushige, Tetsunari, Krause, Michael W., Minnema, Stephanie E., Goszczynski, Barbara, Gaudet, Jeb, Kohara, Yuji, Bossinger, Olaf, Zhao, Yongjun, Khattra, Jaswinder, Hirst, Martin, Jones, Steven J.M., Marra, Marco A., Ruzanov, Peter, Warner, Adam, Zapf, Richard, Moerman, Donald G., Kalb, John M.

“…Starting with SAGE-libraries prepared from C. elegans FAC-sorted embryonic intestine cells (8E–16E cell stage), from total embryos and from purified oocytes,…”
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An integrated strategy to study muscle development and myofilament structure in Caenorhabditis elegans by Meissner, Barbara, Warner, Adam, Wong, Kim, Dube, Nicholas, Lorch, Adam, McKay, Sheldon J, Khattra, Jaswinder, Rogalski, Teresa, Somasiri, Aruna, Chaudhry, Iasha, Fox, Rebecca M, Miller, 3rd, David M, Baillie, David L, Holt, Robert A, Jones, Steven J M, Marra, Marco A, Moerman, Donald G

“…A crucial step in the development of muscle cells in all metazoan animals is the assembly and anchorage of the sarcomere, the essential repeat unit responsible…”
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A network of HSPG core proteins and HS modifying enzymes regulates netrin-dependent guidance of D-type motor neurons in Caenorhabditis elegans by Gysi, Stephan, Rhiner, Christa, Flibotte, Stephane, Moerman, Donald G, Hengartner, Michael O

“…Heparan sulfate proteoglycans (HSPGs) are proteins with long covalently attached sugar side chains of the heparan sulfate (HS) type. Depending on the cellular…”
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Chromosome movements promoted by the mitochondrial protein SPD-3 are required for homology search during Caenorhabditis elegans meiosis by Labrador, Leticia, Barroso, Consuelo, Lightfoot, James, Müller-Reichert, Thomas, Flibotte, Stephane, Taylor, Jon, Moerman, Donald G, Villeneuve, Anne M, Martinez-Perez, Enrique

“…Pairing of homologous chromosomes during early meiosis is essential to prevent the formation of aneuploid gametes. Chromosome pairing includes a step of…”
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The ELT-2 GATA-factor and the global regulation of transcription in the C. elegans intestine by McGhee, James D., Sleumer, Monica C., Bilenky, Mikhail, Wong, Kim, McKay, Sheldon J., Goszczynski, Barbara, Tian, Helen, Krich, Natisha D., Khattra, Jaswinder, Holt, Robert A., Baillie, David L., Kohara, Yuji, Marra, Marco A., Jones, Steven J.M., Moerman, Donald G., Robertson, A. Gordon

“…A SAGE library was prepared from hand-dissected intestines from adult Caenorhabditis elegans, allowing the identification of > 4000 intestinally-expressed…”
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