Dithiothreitol-based protein equalisation in the context of multiple myeloma: Enhancing proteomic analysis and therapeutic insights

Dithiothreitol-based protein equalisation in the context of multiple myeloma: Enhancing proteomic analysis and therapeutic insights

In this study, we employed the dithiothreitol-based protein equalisation technique and analytical proteomics to better understand myeloma diseases by comparing the proteomes of pellets and supernatants formed upon application of DTT on serum samples. The number of unique proteins found in pellets wa...

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Bibliographic Details
Published in:Talanta (Oxford) Vol. 279; p. 126589
Main Authors: Domingos, Ines F., Carvalho, Luis B., Lodeiro, Carlos, Gerivaz, Rita, Prag, Gali, Micaglio, Emanuele, Muchtar, Eli, Santos, Hugo M., Capelo, Jose L.
Format: Journal Article
Language:English
Published: Netherlands Elsevier B.V 01-11-2024
Subjects:
Amyloidosis
Analytical proteomics
Dithiothreitol - chemistry
DTT equalisation
Haematological malignancy
Humans
Multiple myeloma
Multiple Myeloma - drug therapy
Multiple Myeloma - metabolism
Neurotoxicity
Proteome - analysis
Proteome - metabolism
Proteomic profiling
Proteomics - methods
Proteostasis modulation
Haematological malignancy
Neurotoxicity
Analytical proteomics
Proteomic profiling
DTT equalisation
Multiple myeloma
Proteostasis modulation
Amyloidosis
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Summary:In this study, we employed the dithiothreitol-based protein equalisation technique and analytical proteomics to better understand myeloma diseases by comparing the proteomes of pellets and supernatants formed upon application of DTT on serum samples. The number of unique proteins found in pellets was 252 for healthy individuals and 223 for multiple myeloma patients. The comparison of these proteomes showed 97 dysregulated proteins. The unique proteins found for supernatants were 264 for healthy individuals and 235 for multiple myeloma patients. The comparison of these proteomes showed 87 dysregulated proteins. The analytical proteomic comparison of both groups of dysregulated proteins is translated into parallel dysregulated pathways, including chaperone-mediated autophagy and protein folding, addressing potential therapeutic interventions. Future research endeavours in personalised medicine should prioritize refining analytical proteomic methodologies using serum dithiothreitol-based protein equalisation to explore innovative therapeutic strategies. We highlight the advanced insights gained from this analytical strategy in studying multiple myeloma, emphasising its complex nature and the critical role of personalised, targeted analytical techniques in enhancing therapeutic efficacy in personalised medicine. [Display omitted] •DTT-based sample treatment equalizes proteins in serum.•Pelletś and supernatantś proteomes mirror each other.•Up-regulation of amyloid fibre production pathways in MM patients.•Down-regulated protein aggregate management systems in MM patients.•Different drugs are suggested for future MM treatment.
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ISSN:0039-9140
1873-3573
1873-3573
DOI:10.1016/j.talanta.2024.126589