Growth, morphogenesis, and differentiation during mouse prostate development in situ, in renal grafts, and in vitro

BACKGROUND In vitro organ culture and renal grafting of the urogenital sinus (UGS) have both been used as models of prostate development. However, neither has been rigorously examined for its fidelity to replicate the canonical process of prostate differentiation in situ. METHODS We assessed size, m...

Full description

Saved in:

Bibliographic Details
Published in:	The Prostate Vol. 65; no. 4; pp. 390 - 399
Main Authors:	Doles, J.D., Vezina, C.M., Lipinski, R.J., Peterson, R.E., Bushman, W.
Format:	Journal Article
Language:	English
Published:	Hoboken Wiley Subscription Services, Inc., A Wiley Company 01-12-2005 Wiley-Liss
Subjects:	allograft Animals Biological and medical sciences Cell Differentiation - physiology development differentiation Female Gynecology. Andrology. Obstetrics Immunohistochemistry Kidney Transplantation Male Medical sciences Mice Mice, Nude Microscopy, Phase-Contrast Morphogenesis - physiology Nephrology. Urinary tract diseases organ culture Organ Culture Techniques Pregnancy Prostate - anatomy & histology Prostate - cytology Prostate - growth & development Reverse Transcriptase Polymerase Chain Reaction RNA - chemistry RNA - genetics Subrenal Capsule Assay - methods Testosterone - physiology urogenital sinus Andrology Nephrology Growth In situ Homograft Kidney Morphogenesis Development Graft Urogenital system Culture Gynecology Rodentia In vitro allograft Vertebrata Mammalia Urinary system Mouse Animal organ culture Differentiation Urogenital sinus Prostate Organ
Online Access:	Get full text
Tags:	Add Tag No Tags, Be the first to tag this record!

Description
Summary:	BACKGROUND In vitro organ culture and renal grafting of the urogenital sinus (UGS) have both been used as models of prostate development. However, neither has been rigorously examined for its fidelity to replicate the canonical process of prostate differentiation in situ. METHODS We assessed size, morphology, histology, and the mRNA expression of differentiation marker genes of the E14 male mouse UGS grown for 0–28 days as sub‐renal capsule allografts in nude mice or in culture containing androgen and compared these to UGS development in situ. RESULTS Development of grafted tissues was morphologically and histologically similar to development in situ but differentiation occurred more rapidly. UGS growth in organ culture resulted in bud formation, but did not trigger cellular differentiation. However, the potential for differentiation was maintained and could be rescued by grafting tissues into nude mice. CONCLUSIONS In vitro organ culture and renal grafting of UGS tissues may be appropriate models for studying prostatic bud formation, but only grafting is an appropriate model for prostatic differentiation. © 2005 Wiley‐Liss, Inc.
Bibliography:	NIH - No. RO1 DK065303 istex:6858F2E309F1865F608E881BDA77A29F2CCAB3BC ArticleID:PROS20321 NIH - No. RO1 DK052687 J.D. Doles and C.M. Vezina contributed equally to this work. ark:/67375/WNG-1PM9BH7S-1 NIH - No. R37 ES01332 ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 23
ISSN:	0270-4137 1097-0045
DOI:	10.1002/pros.20321