Immunocytochemical identification of carcinomas of unknown primary in serous effusions

Metastases from carcinomas of unknown primary site (CUP) in serous effusion are a common clinical problem. Immunocytochemistry was applied as an adjunct to the cytological diagnosis of metastatic carcinomas in serous effusions. Subjects of this study were 118 pleural, 53 peritoneal, and 9 pericardia...

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Bibliographic Details
Published in:	Diagnostic cytopathology Vol. 33; no. 5; pp. 309 - 315
Main Authors:	Pomjanski, Natalia, Juergen Grote, Hans, Doganay, Peyrüze, Schmiemann, Viola, Buckstegge, Birgit, Böcking, Alfred
Format:	Journal Article
Language:	English
Published:	Hoboken Wiley Subscription Services, Inc., A Wiley Company 01-11-2005
Subjects:	Adult Aged Aged, 80 and over Algorithms ascitic fluid Ascitic Fluid - metabolism Ascitic Fluid - pathology Biomarkers, Tumor - metabolism Carcinoma - diagnosis Carcinoma - metabolism Carcinoma - secondary Female Humans immunocytochemistry Immunohistochemistry Male Middle Aged neoplasms neoplasms, unknown primary Neoplasms, Unknown Primary - diagnosis Neoplasms, Unknown Primary - metabolism Neoplasms, Unknown Primary - pathology pericardial effusion Pericardial Effusion - diagnosis Pericardial Effusion - pathology pleural effusion Pleural Effusion, Malignant - diagnosis Pleural Effusion, Malignant - pathology unknown primary
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Summary:	Metastases from carcinomas of unknown primary site (CUP) in serous effusion are a common clinical problem. Immunocytochemistry was applied as an adjunct to the cytological diagnosis of metastatic carcinomas in serous effusions. Subjects of this study were 118 pleural, 53 peritoneal, and 9 pericardial effusions from 180 patients routinely investigated in the Institute of Cytopathology. Specimens were primarily stained according to Papanicolaou (Pap). The avidin‐biotin‐complex method (ABC) was secondarily applied for the visualization of immunologic reactions. We have used a panel of six monoclonal antibodies (CK 5/6, CK 7, CK 20, CA 125, TTF‐1, and cdx 2) so as to identify the primary tumor site of metastatic carcinoma cells in serous effusions. Applying an algorithm of immunocytochemical marker constellations, we were able to correctly diagnose primary tumor sites in 86 of 101 (85.1%) patients with CUP syndromes. The best result was achieved for the identification of metastatic carcinomas of the ovaries (94.7%) and the lungs (88.1%). We established an algorithm comprising six immunocytochemical markers that enabled a correct diagnosis of primary tumor sites in 85.1%. The panel studied could be useful in diagnostic routine for the identification of primary tumors of unknown origin metastatic to the serous membranes. Diagn. Cytopathol. 2005;33:309–315. © 2005 Wiley‐Liss, Inc.
Bibliography:	ark:/67375/WNG-8V1ZR8G5-X istex:A146607A0AC0B61F0F496A3741B60822F69FD5E2 ArticleID:DC20393 ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 23
ISSN:	8755-1039 1097-0339
DOI:	10.1002/dc.20393