Search Results - "Doench, J."

siRNAs can function as miRNAs by Doench, John G, Petersen, Christian P, Sharp, Phillip A

“…With the discovery of RNA interference (RNAi) and related phenomena, new regulatory roles attributed to RNA continue to emerge. Here we show, in mammalian…”
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Specificity of microRNA target selection in translational repression by Doench, John G, Sharp, Phillip A

“…MicroRNAs (miRNAs) are a class of noncoding RNAs found in organisms as evolutionarily distant as plants and mammals, yet most of the mRNAs they regulate are…”
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Screens using RNAi and cDNA expression as surrogates for genetics in mammalian tissue culture cells by Pearlberg, J, Degot, S, Endege, W, Park, J, Davies, J, Gelfand, E, Sawyer, J, Conery, A, Doench, J, Li, W, Gonzalez, L, Boyce, F M, Brizuela, L, Labaer, J, Grueneberg, D, Harlow, E

“…We have developed methods for the automation of transfection-grade DNA preparation, high-throughput retroviral preparation, and highly parallel phenotypic…”
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Functional Genetic Screens to Dissect the Essentialome of Disease by Woods, K., Dettwiler, M., Inglebert, M., Maurizio, M., Olias, P., Doench, J., Rottenberg, S.

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Genome-scale genetic screening identifies PRMT1 as a critical vulnerability in castration-resistant prostate cancer by Tang, S., Metaferia, N., Nogueira, M., Gelbard, M., Alaiwi, S. Abou, Seo, J.H., Hwang, J., Strathdee, C., Baca, S., Li, J., Abuhammad, S., Zhang, X., Doench, J., Hahn, W., Takeda, D., Freedman, M., Choi, P., Viswanathan, S.

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Targeted shRNA screening identified critical roles of pleckstrin-2 in erythropoiesis by Zhao, Baobing, Keerthivasan, Ganesan, Mei, Yang, Yang, Jing, McElherne, James, Wong, Piu, Doench, John G, Feng, Gang, Root, David E, Ji, Peng

“…Differentiation of erythroblasts to mature red blood cells involves dynamic changes of the membrane and cytoskeleton networks that are not fully characterized…”
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Distribution of physiological adult lactase phenotypes, lactose absorber and malabsorber, in Germany by Flatz, G, Howell, J N, Doench, J, Flatz, S D

“…A total of 1805 apparently healthy, adult and adolescent Germans (1572 males and 233 females with a mean age of 20.3 years) were examined for lactose…”
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CRISPR Activation Screen for Drivers of MM Cell Proliferation by Tang, Huihui, De Matos Simoes, Ricardo, Shirasaki, Ryosuke, Dashevsky, Olga, Glassner, Brian, Downey-Kopyscinski, Sondra L., Gandolfi, Sara, Sheffer, Michal, Bariteau, Megan, Doench, John G, Mitsiades, Constantine S.

“…CRISPR/Cas9-based gene editing has become a powerful tool for loss-of-function (LOF) studies and has allowed us to systematically interrogate the function of…”
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CRISPR Activation Screen for HDAC Inhibitor Resistance by Tang, Huihui, De Matos Simoes, Ricardo, Shirasaki, Ryosuke, Dashevsky, Olga, Glassner, Brian, Downey-Kopyscinski, Sondra L., Gandolfi, Sara, Sheffer, Michal, Bariteau, Megan, Doench, John G, Mitsiades, Constantine S.

“…Panobinostat is an oral broad-spectrum histone deacetylase inhibitor that alters gene expression via epigenetic mechanisms and function of key proteins through…”
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CRISPR-Based Dual-Knockout Screens Reveals Network of Interactions between Key Dependencies for Myeloma Cells and Highlights IRF4 As a Central Regulator of Myeloma Cell Behavior by Downey-Kopyscinski, Sondra L., De Matos Simoes, Ricardo, Shirasaki, Ryosuke, Dashevsky, Olga, Glassner, Brian, Bare, Olivia, Doench, John G, Mitsiades, Constantine S.

“…As multiple myeloma (MM) remains incurable, development of more effective novel therapies will require a deeper understanding of which genes, pathways and…”
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RNA interference of Xenopus NMDAR NR1 in vitro and in vivo by Miskevich, Frank, Doench, John G., Townsend, Matthew T., Sharp, Phillip A., Constantine-Paton, Martha

“…Here, we present a short interfering RNA (siRNA) application that reduces the expression of NR1 the obligate subunit of the NMDA receptor (NMDAR) and virtually…”
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