Search Results - "Dodani, Sheel C."

Discovery and Characterization of a Naturally Occurring, Turn-On Yellow Fluorescent Protein Sensor for Chloride by Tutol, Jasmine N, Peng, Weicheng, Dodani, Sheel C

“…Fluorescent proteins have been extensively engineered and applied as optical indicators for chloride in a variety of biological contexts. Surprisingly, given…”
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Discovery of a regioselectivity switch in nitrating P450s guided by molecular dynamics simulations and Markov models by Dodani, Sheel C., Kiss, Gert, Cahn, Jackson K. B., Su, Ye, Pande, Vijay S., Arnold, Frances H.

“…The dynamic motions of protein structural elements, particularly flexible loops, are intimately linked with diverse aspects of enzyme catalysis. Engineering of…”
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Identification of mNeonGreen as a pH‐Dependent, Turn‐On Fluorescent Protein Sensor for Chloride by Tutol, Jasmine N., Kam, Hiu C., Dodani, Sheel C.

“…Chloride‐sensitive fluorescent proteins generated from laboratory evolution have a characteristic tyrosine residue that interacts with a chloride ion and…”
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Unlocking chloride sensing in the red at physiological pH with a fluorescent rhodopsin-based host by Phelps, Shelby M, Tutol, Jasmine N, Advani, Deeya, Peng, Weicheng, Dodani, Sheel C

“…Chloride is a vital ion for all forms of life. Protein-based fluorescent biosensors can enable researchers to visualize chloride in cells but remain…”
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Subcellular metal imaging identifies dynamic sites of Cu accumulation in Chlamydomonas by Hong-Hermesdorf, Anne, Miethke, Marcus, Gallaher, Sean D, Kropat, Janette, Dodani, Sheel C, Chan, Jefferson, Barupala, Dulmini, Domaille, Dylan W, Shirasaki, Dyna I, Loo, Joseph A, Weber, Peter K, Pett-Ridge, Jennifer, Stemmler, Timothy L, Chang, Christopher J, Merchant, Sabeeha S

“…A collection of chemical tools and spectroscopic techniques demonstrate that Zn availability influences Cu + storage and localization in the green alga…”
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Copper is an endogenous modulator of neural circuit spontaneous activity by Dodani, Sheel C., Firl, Alana, Chan, Jefferson, Nam, Christine I., Aron, Allegra T., Onak, Carl S., Ramos-Torres, Karla M., Paek, Jaeho, Webster, Corey M., Feller, Maria B., Chang, Christopher J.

“…For reasons that remain insufficiently understood, the brain requires among the highest levels of metals in the body for normal function. The traditional…”
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Calcium-dependent copper redistributions in neuronal cells revealed by a fluorescent copper sensor and X-ray fluorescence microscopy by Dodani, Sheel C, Domaille, Dylan W, Nam, Christine I, Miller, Evan W, Finney, Lydia A, Vogt, Stefan, Chang, Christopher J

“…Dynamic fluxes of s-block metals like potassium, sodium, and calcium are of broad importance in cell signaling. In contrast, the concept of mobile transition…”
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Directed Evolution of a Bright Near-Infrared Fluorescent Rhodopsin Using a Synthetic Chromophore by Herwig, Lukas, Rice, Austin J, Bedbrook, Claire N, Zhang, Ruijie K, Lignell, Antti, Cahn, Jackson K B, Renata, Hans, Dodani, Sheel C, Cho, Inha, Cai, Long, Gradinaru, Viviana, Arnold, Frances H

“…By engineering a microbial rhodopsin, Archaerhodopsin-3 (Arch), to bind a synthetic chromophore, merocyanine retinal, in place of the natural chromophore…”
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The Arabidopsis COPT6 transport protein functions in copper distribution under copper-deficient conditions by Garcia-Molina, Antoni, Andrés-Colás, Nuria, Perea-García, Ana, Neumann, Ulla, Dodani, Sheel C, Huijser, Peter, Peñarrubia, Lola, Puig, Sergi

“…Copper (Cu), an essential redox active cofactor, participates in fundamental biological processes, but it becomes highly cytotoxic when present in excess…”
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Non-natural Olefin Cyclopropanation Catalyzed by Diverse Cytochrome P450s and Other Hemoproteins by Heel, Thomas, McIntosh, John A., Dodani, Sheel C., Meyerowitz, Joseph T., Arnold, Frances H.

“…Recent work has shown that engineered variants of cytochrome P450BM3 (CYP102A1) efficiently catalyze non‐natural reactions, including carbene and nitrene…”
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Reaction-based small-molecule fluorescent probes for chemoselective bioimaging by Chan, Jefferson, Dodani, Sheel C., Chang, Christopher J.

“…The complexity of living systems makes attempts to gain a molecular-level understanding of them a unique and inspiring challenge. This Review summarizes…”
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Structural, Functional, and Spectroscopic Characterization of the Substrate Scope of the Novel Nitrating Cytochrome P450 TxtE by Dodani, Sheel C., Cahn, Jackson K. B., Heinisch, Tillmann, Brinkmann-Chen, Sabine, McIntosh, John A., Arnold, Frances H.

“…A novel cytochrome P450 enzyme, TxtE, was recently shown to catalyze the direct aromatic nitration of L‐tryptophan. This unique chemistry inspired us to ask…”
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Engineering the ChlorON Series: Turn-On Fluorescent Protein Sensors for Imaging Labile Chloride in Living Cells by Tutol, Jasmine N., Ong, Whitney S. Y., Phelps, Shelby M., Peng, Weicheng, Goenawan, Helen, Dodani, Sheel C.

“…Beyond its role as the “queen of electrolytes”, chloride can also serve as an allosteric regulator or even a signaling ion. To illuminate this essential anion…”
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A Turn-On Fluorescent Sensor for Detecting Nickel in Living Cells by Dodani, Sheel C, He, Qiwen, Chang, Christopher J

“…We present the synthesis and properties of Nickelsensor-1 (NS1), a new water-soluble, turn-on fluorescent sensor that is capable of selectively responding to…”
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A Targetable Fluorescent Sensor Reveals That Copper-Deficient SCO1 and SCO2 Patient Cells Prioritize Mitochondrial Copper Homeostasis by Dodani, Sheel C, Leary, Scot C, Cobine, Paul A, Winge, Dennis R, Chang, Christopher J

“…We present the design, synthesis, spectroscopy, and biological applications of Mitochondrial Coppersensor-1 (Mito-CS1), a new type of targetable fluorescent…”
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The mammalian phosphate carrier SLC25A3 is a mitochondrial copper transporter required for cytochrome c oxidase biogenesis by Boulet, Aren, Vest, Katherine E., Maynard, Margaret K., Gammon, Micah G., Russell, Antoinette C., Mathews, Alexander T., Cole, Shelbie E., Zhu, Xinyu, Phillips, Casey B., Kwong, Jennifer Q., Dodani, Sheel C., Leary, Scot C., Cobine, Paul A.

“…Copper is required for the activity of cytochrome c oxidase (COX), the terminal electron-accepting complex of the mitochondrial respiratory chain. The likely…”
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Transcriptome Sequencing Identifies SPL7-Regulated Copper Acquisition Genes FRO4/FRO5 and the Copper Dependence of Iron Homeostasis in Arabidopsis by Bemal, Maria, Casero, David, Singh, Vasantika, Wilson, Grandon T., Grande, Arne, Yang, Huijun, Dodani, Sheel, Pellegrini, Matteo, Huijser, Peter, Connolly, Erin L., Merchant, Sabeeha S., Krämer, Ute

“…The transition metal copper (Cu) is essential for all living organisms but is toxic when present in excess. To identify Cu deficiency responses…”
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The design and evolution of fluorescent protein-based sensors for monoatomic ions in biology by Baek, Kiheon, Ji, Ke, Peng, Weicheng, Liyanaarachchi, Sureshee M, Dodani, Sheel C

“…Abstract Living cells rely on a finely tuned symphony of inorganic ion gradients composed of both cations and anions. This delicate balance is maintained by…”
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Supramolecular Reinforcement of a Large-Pore 2D Covalent Organic Framework by Diwakara, Shashini D, Ong, Whitney S. Y, Wijesundara, Yalini H, Gearhart, Robert L, Herbert, Fabian C, Fisher, Sarah G, McCandless, Gregory T, Alahakoon, Sampath B, Gassensmith, Jeremiah J, Dodani, Sheel C, Smaldone, Ronald A

“…Two-dimensional covalent organic frameworks (2D-COFs) are a class of crystalline porous organic polymers that consist of covalently linked, two-dimensional…”
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Biophysical and in silico characterization of NrtA: a protein-based host for aqueous nitrate and nitrite recognition by Ji, Ke, Baek, Kiheon, Peng, Weicheng, Alberto, Kevin A, Torabifard, Hedieh, Nielsen, Steven O, Dodani, Sheel C

“…Nitrate and nitrite are key components of the global nitrogen cycle. As such, Nature has evolved proteins as biological supramolecular hosts for the…”
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