Search Results - "Dobberstein, B."

An alternative protein targeting pathway in Escherichia coli: studies on the role of FtsY by Luirink, J., Hagen‐Jongman, C.M., Weijden, C.C., Oudega, B., High, S., Dobberstein, B., Kusters, R.

“…In Escherichia coli, a signal recognition particle (SRP) has been identified which binds specifically to the signal sequence of presecretory proteins and which…”
Get full text

Site-specific photocross-linking reveals that Sec61p and TRAM contact different regions of a membrane-inserted signal sequence by High, S, Martoglio, B, Görlich, D, Andersen, S S, Ashford, A J, Giner, A, Hartmann, E, Prehn, S, Rapoport, T A, Dobberstein, B

“…A chemically charged amber suppressor tRNA was used to introduce the photoactivatable amino acid (Tmd)Phe at a selected position within the signal sequence of…”
Get full text

A complex of the signal sequence binding protein and the SRP RNA promotes translocation of nascent proteins by Hauser, S., Bacher, G., Dobberstein, B., Lütcke, H.

“…Translocation of proteins across the endoplasmic reticulum membrane is initiated by the signal recognition particle (SRP), a cytoplasmic ribonucleoprotein…”
Get full text

The methionine‐rich domain of the 54 kDa subunit of signal recognition particle is sufficient for the interaction with signal sequences by Lütcke, H., High, S., Römisch, K., Ashford, A.J., Dobberstein, B.

“…The signal recognition particle (SRP) binds to signal sequences when they emerge from a translating ribosome and targets the complex of ribosome, nascent chain…”
Get full text

Requirements for the Membrane Insertion of Signal-Anchor Type Proteins by High, Stephen, Flint, Nicholas, Dobberstein, Bernhard

“…Proteins which are inserted and anchored in the membrane of the ER by an uncleaved signal-anchor sequence can assume two final orientations. Type I…”
Get full text

Primary structure of the gene for the murine Ia antigen‐associated invariant chains (Ii). An alternatively spliced exon encodes a cysteine‐rich domain highly homologous to a repetitive sequence of thyroglobulin by Koch, N., Lauer, W., Habicht, J., Dobberstein, B.

“…The gene for murine Ia‐associated invariant (Ii) chains (Ii31 and Ii41) was characterized by sequence analysis. The gene extends over approximately 9 kb and is…”
Get full text

Signal sequences: more than just greasy peptides by Martoglio, Bruno, Dobberstein, Bernhard

“…Export signal sequences target newly synthesized proteins to the endoplasmic reticulum of eukaryotic cells and the plasma membrane of bacteria. All signal…”
Get full text

Common Principles of Protein Translocation Across Membranes by Schatz, Gottfried, Dobberstein, Bernhard

“…Most major systems that transport proteins across a membrane share the following features: an amino-terminal transient signal sequence on the transported…”
Get full text

A novel in vitro transcription‐translation system: accurate and efficient synthesis of single proteins from cloned DNA sequences by Stueber, D., Ibrahimi, I., Cutler, D., Dobberstein, B., Bujard, H.

“…A system is described which permits the efficient synthesis of single proteins in vitro. The essential element in this expression system is a strong promoter…”
Get full text

Mouse histocompatibility genes: structure and organisation of a Kd gene by Kvist, S., Roberts, L., Dobberstein, B.

“…The gene coding for the mouse H‐2Kd antigen has been isolated by using a K‐locus specific cDNA probe. The complete nucleotide sequence of the gene reveals…”
Get full text

Transfer of Proteins across Membranes. I. Presence of Proteolytically Processed and Unprocessed Nascent Immunoglobulin Light Chains on Membrane-Bound Ribosomes of Murine Myeloma by Blobel, Günter, Dobberstein, Bernhard

“…Fractionation of MOPC 41 DL-1 tumors revealed that the mRNA for the light chain of immunoglobulin is localized exclusively in membrane-bound ribosomes. It was…”
Get full text

Transfer of proteins across membranes. II. Reconstitution of functional rough microsomes from heterologous components by Blobel, G, Dobberstein, B

“…The data presented in this paper demonstrate that native small ribosomal subunits from reticulocytes (containing initiation factors) and large ribosomal…”
Get full text

The Signal Sequence Receptor Has a Second Subunit and Is Part of a Translocation Complex in the Endoplasmic Reticulum as Probed by Bifunctional Reagents by Görlich, Dirk, Prehn, Siegfried, Hartmann, Enno, Herz, Joachim, Otto, Albrecht, Kraft, Regine, Wiedmann, Martin, Knespel, Siegne, Dobberstein, Bernhard, Rapoport, Tom A.

“…Bifunctional cross-linking reagents were used to probe the protein environment in the ER membrane of the signal sequence receptor (SSR), a 34-kD integral…”
Get full text

Protein insertion into the membrane of the endoplasmic reticulum: the architecture of the translocation site by Martoglio, B, Dobberstein, B

Get more information

Structural Requirements for Membrane Assembly of Proteins Spanning the Membrane Several Times by Lipp, Joachim, Flint, Nicholas, Haeuptle, Marie-Theres, Dobberstein, Bernhard

“…We have investigated the structural requirements for the biogenesis of proteins spanning the membrane several times. Proteins containing various combinations…”
Get full text

Signal and Membrane Anchor Functions Overlap in the Type II Membrane Protein IγCAT by Lipp, Joachim, Dobberstein, Bernhard

“…IγCAT is a hybrid protein that inserts into the membrane of the endoplasmic reticulum as a type II membrane protein. These proteins span the membrane once and…”
Get full text

Association of immunoglobulin‐like transcript 6 deficiency with Sjögren's syndrome by Kabalak, G., Dobberstein, S. B., Matthias, T., Reuter, S., The, Y.‐H., Dörner, T., Schmidt, R. E., Witte, T.

“…Objective The immunoglobulin‐like transcript (ILT) family is located in chromosomal region 19q13 and consists of a group of activating and inhibitory…”
Get full text

Signal Recognition Particle-Dependent Membrane Insertion of Mouse Invariant Chain: A Membrane-Spanning Protein with a Cytoplasmically Exposed Amino Terminus by Lipp, Joachim, Dobberstein, Bernhard

“…Invariant (Ii) chain is a membrane-spanning protein that is found associated intracellularly with class II histocompatibility antigens. In the endoplasmic…”
Get full text

Isolation and characterization of a cDNA clone encoding the 19 kDa protein of signal recognition particle (SRP): expression and binding to 7SL RNA by Lingelbach, K, Zwieb, C, Webb, J R, Marshallsay, C, Hoben, P J, Walter, P, Dobberstein, B

“…Signal recognition particle (SRP) consists of a 7SL RNA molecule and 6 protein subunits. We have isolated and characterized cDNA clones from human liver which…”
Get full text

A Tripartite Structure of the Signals That Determine Protein Insertion into the Endoplasmic Reticulum Membrane by Haeuptle, Marie-Theres, Flint, Nicholas, Gough, Nicholas M., Dobberstein, Bernhard

“…Multilineage colony stimulating factor is a secretory protein with a cleavable signal sequence that is unusually long and hydrophobic. Using molecular cloning…”
Get full text