Field evaluation of Crystal VC® Rapid Dipstick test for cholera during a cholera outbreak in Guinea-Bissau

Field evaluation of Crystal VC® Rapid Dipstick test for cholera during a cholera outbreak in Guinea-Bissau

To evaluate performance characteristics and ease of use of the new commercially available Crystal VC® Rapid Dipstick (VC) test (Span Diagnostics, India) for Vibrio cholerae O1 and O139. Whole stool was collected from patients presenting to a hospital cholera ward during a 2008 epidemic in Guinea-Bis...

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Bibliographic Details
Published in:Tropical medicine & international health Vol. 14; no. 9; pp. 1117 - 1121
Main Authors: Harris, Julie R, Cavallaro, Elizabeth C, de Nóbrega, Aglaêr A, dos S. Barrado, Jean C.B, Bopp, Cheryl, Parsons, Michele B, Djalo, Djulde, Fonseca, Fatima G. da S, Ba, Umaro, Semedo, Agostinho, Sobel, Jeremy, Mintz, Eric D
Format: Journal Article
Language:English
Published: Oxford, UK Oxford, UK : Blackwell Publishing Ltd 01-09-2009
Blackwell Publishing Ltd
Blackwell
Subjects:
Adolescent
Adult
Aged
Bacterial diseases
Biological and medical sciences
Child
Child, Preschool
Cholera
Cholera - diagnosis
Cholera - epidemiology
Diagnostic tests
diarrhea
diarrhoea
dipstick
Feces - microbiology
Female
General aspects
Guinea-Bissau - epidemiology
Guinea‐Bissau
Human bacterial diseases
Humans
Infectious diseases
Male
Medical sciences
Middle Aged
Predictive Value of Tests
rapid test
Reagent Kits, Diagnostic
Sensitivity and Specificity
Tropical bacterial diseases
Vibrio
Vibrio cholerae
Vibrio cholerae O1 - isolation & purification
Young Adult
Guinea-Bissau
Africa
Guinea Bissau
India
Epidemic
rapid test
Diarrhea
dipstick
diarrhoea
Infection
Rapid technique
Guinea-Bissau
Reagent strip
Bacteriosis
Bacteria
Digestive diseases
Intestinal disease
Vibrionaceae
Tropical medicine
Cholera
Vibrio
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Summary:To evaluate performance characteristics and ease of use of the new commercially available Crystal VC® Rapid Dipstick (VC) test (Span Diagnostics, India) for Vibrio cholerae O1 and O139. Whole stool was collected from patients presenting to a hospital cholera ward during a 2008 epidemic in Guinea-Bissau. The VC test on stool samples was conducted on-site; samples were subsequently stored in Cary-Blair transport media and sent to the Centers for Disease Control and Prevention for diagnostic testing by culture and polymerase chain reaction (PCR). In addition, four local laboratory technicians who were unfamiliar with the test were provided with stool samples, the VC test kit, and simple written instructions and asked to perform the test and interpret results. A total of 101 stool specimens were collected and tested. Compared with PCR, the test was 97% sensitive and 71-76% specific. Laboratory technicians in Bissau performed the test and interpreted results correctly using only simple written instructions. The VC test may be useful for cholera diagnosis in outbreak situations where laboratory capacity is limited.
Bibliography:http://dx.doi.org/10.1111/j.1365-3156.2009.02335.x
ObjectType-Article-1
SourceType-Scholarly Journals-1
ObjectType-Feature-2
content type line 23
ISSN:1360-2276
1365-3156
DOI:10.1111/j.1365-3156.2009.02335.x