Search Results - "Dixon, Nicholas E."

NMR Spectroscopic Assignment of Backbone and Side-Chain Protons in Fully Protonated Proteins: Microcrystals, Sedimented Assemblies, and Amyloid Fibrils by Stanek, Jan, Andreas, Loren B., Jaudzems, Kristaps, Cala, Diane, Lalli, Daniela, Bertarello, Andrea, Schubeis, Tobias, Akopjana, Inara, Kotelovica, Svetlana, Tars, Kaspars, Pica, Andrea, Leone, Serena, Picone, Delia, Xu, Zhi-Qiang, Dixon, Nicholas E., Martinez, Denis, Berbon, Mélanie, El Mammeri, Nadia, Noubhani, Abdelmajid, Saupe, Sven, Habenstein, Birgit, Loquet, Antoine, Pintacuda, Guido

“…We demonstrate sensitive detection of alpha protons of fully protonated proteins by solid‐state NMR spectroscopy with 100–111 kHz magic‐angle spinning (MAS)…”
Get full text

Architecture and conservation of the bacterial DNA replication machinery, an underexploited drug target by Robinson, Andrew, Causer, Rebecca J, Dixon, Nicholas E

“…New antibiotics with novel modes of action are required to combat the growing threat posed by multi-drug resistant bacteria. Over the last decade, genome…”
Get more information

Single-molecule visualization of stalled replication-fork rescue by the Escherichia coli Rep helicase by Whinn, Kelsey S, Xu, Zhi-Qiang, Jergic, Slobodan, Sharma, Nischal, Spenkelink, Lisanne M, Dixon, Nicholas E, van Oijen, Antoine M, Ghodke, Harshad

“…Abstract Genome duplication occurs while the template DNA is bound by numerous DNA-binding proteins. Each of these proteins act as potential roadblocks to the…”
Get full text

Single-molecule visualization of fast polymerase turnover in the bacterial replisome by Lewis, Jacob S, Spenkelink, Lisanne M, Jergic, Slobodan, Wood, Elizabeth A, Monachino, Enrico, Horan, Nicholas P, Duderstadt, Karl E, Cox, Michael M, Robinson, Andrew, Dixon, Nicholas E, van Oijen, Antoine M

“…The DNA replication machinery has been used as a road map to uncover design rules that enable DNA duplication with high efficiency and fidelity. Although the…”
Get full text

Structural characterisation of the complete cycle of sliding clamp loading in Escherichia coli by Xu, Zhi-Qiang, Jergic, Slobodan, Lo, Allen T. Y., Pradhan, Alok C., Brown, Simon H. J., Bouwer, James C., Ghodke, Harshad, Lewis, Peter J., Tolun, Gökhan, Oakley, Aaron J., Dixon, Nicholas E.

“…Ring-shaped DNA sliding clamps are essential for DNA replication and genome maintenance. Clamps need to be opened and chaperoned onto DNA by clamp loader…”
Get full text

Nuclease dead Cas9 is a programmable roadblock for DNA replication by Whinn, Kelsey S., Kaur, Gurleen, Lewis, Jacob S., Schauer, Grant D., Mueller, Stefan H., Jergic, Slobodan, Maynard, Hamish, Gan, Zhong Yan, Naganbabu, Matharishwan, Bruchez, Marcel P., O’Donnell, Michael E., Dixon, Nicholas E., van Oijen, Antoine M., Ghodke, Harshad

“…Limited experimental tools are available to study the consequences of collisions between DNA-bound molecular machines. Here, we repurpose a catalytically…”
Get full text

Backbone Assignment of Fully Protonated Solid Proteins by 1H Detection and Ultrafast Magic-Angle-Spinning NMR Spectroscopy by Marchetti, Alessandro, Jehle, Stefan, Felletti, Michele, Knight, Michael J., Wang, Yao, Xu, Zhi-Qiang, Park, Ah Young, Otting, Gottfried, Lesage, Anne, Emsley, Lyndon, Dixon, Nicholas E., Pintacuda, Guido

“…Narrow 1H NMR linewidths can be obtained for fully protonated protein samples in the solid state by using ultrafast magic‐angle spinning (60 kHz). Medium‐size…”
Get full text

Polymerase exchange on single DNA molecules reveals processivity clamp control of translesion synthesis by Kath, James E., Jergic, Slobodan, Heltzel, Justin M. H., Jacob, Deena T., Dixon, Nicholas E., Sutton, Mark D., Walker, Graham C., Loparo, Joseph J.

“…Translesion synthesis (TLS) by Y-family DNA polymerases alleviates replication stalling at DNA damage. Ring-shaped processivity clamps play a critical but…”
Get full text

Replisome speed determines the efficiency of the Tus−Ter replication termination barrier by Elshenawy, Mohamed M., Jergic, Slobodan, Xu, Zhi-Qiang, Sobhy, Mohamed A., Takahashi, Masateru, Oakley, Aaron J., Dixon, Nicholas E., Hamdan, Samir M.

“…The Tus– Ter termination site of Escherichia coli is not completely efficient in stopping DNA replication, with about half of replisomes bypassing this…”
Get full text

Roquin binds microRNA-146a and Argonaute2 to regulate microRNA homeostasis by Srivastava, Monika, Duan, Guowen, Kershaw, Nadia J., Athanasopoulos, Vicki, Yeo, Janet H. C., Ose, Toyoyuki, Hu, Desheng, Brown, Simon H. J., Jergic, Slobodan, Patel, Hardip R., Pratama, Alvin, Richards, Sashika, Verma, Anil, Jones, E. Yvonne, Heissmeyer, Vigo, Preiss, Thomas, Dixon, Nicholas E., Chong, Mark M. W., Babon, Jeffrey J., Vinuesa, Carola G.

“…Roquin is an RNA-binding protein that prevents autoimmunity and inflammation via repression of bound target mRNAs such as inducible costimulator ( Icos ). When…”
Get full text

Synthesis and properties of crosslinked recombinant pro-resilin by Huson, Mickey G, Maxwell, Jane M, Dixon, Nicholas E, Pearson, Roger D, Carr, Andrew G, Wong, Darren C. C, Liyou, Nancy E, Elvin, Christopher M, Vuocolo, Tony, Merritt, David J

“…Resilin is a member of a family of elastic proteins that includes elastin, as well as gluten, gliadin, abductin and spider silks. Resilin is found in…”
Get full text

A direct proofreader-clamp interaction stabilizes the Pol III replicase in the polymerization mode by Jergic, Slobodan, Horan, Nicholas P, Elshenawy, Mohamed M, Mason, Claire E, Urathamakul, Thitima, Ozawa, Kiyoshi, Robinson, Andrew, Goudsmits, Joris M H, Wang, Yao, Pan, Xuefeng, Beck, Jennifer L, van Oijen, Antoine M, Huber, Thomas, Hamdan, Samir M, Dixon, Nicholas E

“…Processive DNA synthesis by the αεθ core of the Escherichia coli Pol III replicase requires it to be bound to the β 2 clamp via a site in the α polymerase…”
Get full text

Single-molecule studies of fork dynamics in Escherichia coli DNA replication by van Oijen, Antoine M, Hamdan, Samir M, Tanner, Nathan A, Schaeffer, Patrick M, Loscha, Karin V, Dixon, Nicholas E, Jergic, Slobodan

“…We present single-molecule studies of the Escherichia coli replication machinery. We visualize individual E. coli DNA polymerase III (Pol III) holoenzymes…”
Get full text

Strand separation establishes a sustained lock at the Tus–Ter replication fork barrier by Berghuis, Bojk A, Dulin, David, Xu, Zhi-Qiang, van Laar, Theo, Cross, Bronwen, Janissen, Richard, Jergic, Slobodan, Dixon, Nicholas E, Depken, Martin, Dekker, Nynke H

“…Tus protein bound to Ter sites on circular bacterial chromosomes provides a way to avoid random crashes of opposing replication forks. DNA-unzipping…”
Get full text

High-yield cell-free protein synthesis for site-specific incorporation of unnatural amino acids at two sites by Ozawa, Kiyoshi, Loscha, Karin V., Kuppan, Kekini V., Loh, Choy Theng, Dixon, Nicholas E., Otting, Gottfried

“…Cell-free synthesis. [Display omitted] ► Cell-free synthesis affords high protein yields with unnatural amino acids. ► 4-(Trifluoromethyl)phenylalanine…”
Get full text

Multiple classes and isoforms of the RNA polymerase recycling motor protein HelD by Larsen, Joachim S., Miller, Michael, Oakley, Aaron J., Dixon, Nicholas E., Lewis, Peter J.

“…Efficient control of transcription is essential in all organisms. In bacteria, where DNA replication and transcription occur simultaneously, the replication…”
Get full text

Single-Molecule Insights Into the Dynamics of Replicative Helicases by Spinks, Richard R., Spenkelink, Lisanne M., Dixon, Nicholas E., van Oijen, Antoine M.

“…Helicases are molecular motors that translocate along single-stranded DNA and unwind duplex DNA. They rely on the consumption of chemical energy from…”
Get full text

Essential Biological Processes of an Emerging Pathogen: DNA Replication, Transcription, and Cell Division in Acinetobacter spp by ROBINSON, Andrew, BRZOSKA, Anthony J, TURNER, Kylie M, WITHERS, Ryan, HARRY, Elizabeth J, LEWIS, Peter J, DIXON, Nicholas E

“…Article Usage Stats Services MMBR Citing Articles Google Scholar PubMed Related Content Social Bookmarking CiteULike Delicious Digg Facebook Google+ Mendeley…”
Get full text

Structure Determination of Protein−Ligand Complexes by Transferred Paramagnetic Shifts by John, Michael, Pintacuda, Guido, Park, Ah Young, Dixon, Nicholas E, Otting, Gottfried

“…Rational drug design depends on the knowledge of the three-dimensional (3D) structure of complexes between proteins and lead compounds of low molecular weight…”
Get full text

Cell-Free Transcription/Translation from PCR-Amplified DNA for High-Throughput NMR Studies by Wu, Peter S. C., Ozawa, Kiyoshi, Lim, Siew P., Vasudevan, Subhash G., Dixon, Nicholas E., Otting, Gottfried

“…Quick and clean: Excellent protein yields in cell‐free protein synthesis can be obtained by using polymerase chain reaction (PCR) amplified DNA templates,…”
Get full text