Electrophoresis PDMS/glass chips with continuous on-chip derivatization and analysis of amino acids using naphthalene-2,3-dicarboxaldehyde as fluorogenic agent

In this work, we developed a PDMS electrophoresis device able to carry out on-chip derivatization and quantification of amino acids (AAs) using naphthalene-2,3-dicarboxaldehyde (NDA) as a fluorogenic agent. A chemical modification of the PDMS surface was found compulsory to achieve the derivatizatio...

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Bibliographic Details
Published in:	Analytica chimica acta Vol. 609; no. 2; pp. 215 - 222
Main Authors:	Yassine, O., Morin, P., Dispagne, O., Renaud, L., Denoroy, L., Kleimann, P., Faure, K., Rocca, J.-L., Ouaini, N., Ferrigno, R.
Format:	Journal Article
Language:	English
Published:	Amsterdam Elsevier B.V 25-02-2008 Elsevier
Subjects:	Amino acids Amino Acids - analysis Amino Acids - chemistry Analytical chemistry Animals Capillary electrophoresis microchip Chemistry Chromatographic methods and physical methods associated with chromatography Derivatization Dimethylpolysiloxanes - chemistry Electrophoresis - instrumentation Electrophoresis - methods Electrophoresis, Microchip - instrumentation Electrophoresis, Microchip - methods Exact sciences and technology Fluorescent Dyes - analysis Fluorescent Dyes - chemistry Glass Hippocampus - chemistry Hippocampus - metabolism Laser-induced fluorescence detection Microfluidic Naphthalenes - analysis Naphthalenes - chemistry Other chromatographic methods Rats Spectrometric and optical methods Amino acids Capillary electrophoresis microchip Laser-induced fluorescence detection Derivatization Microfluidic Dimethylsiloxane polymer Fluid mechanics Capillary electrophoresis Fluorogenic reagent Laser induced fluorescence Glass System on a chip Glycine Fluorescence spectrometry Biological compound Chemical modification Naphthalene Aminoacid Detection limit Microfluidics Quantitative analysis Atomic absorption spectrometry
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Summary:	In this work, we developed a PDMS electrophoresis device able to carry out on-chip derivatization and quantification of amino acids (AAs) using naphthalene-2,3-dicarboxaldehyde (NDA) as a fluorogenic agent. A chemical modification of the PDMS surface was found compulsory to achieve the derivatization of AAs with NDA and a limit of detection (LOD) of 40 nM was reached for glycine. Finally, we suggested the applicability of this microdevice for the analysis of real biological samples such as a rat hippocampus microdialysate.
Bibliography:	ObjectType-Article-2 SourceType-Scholarly Journals-1 ObjectType-Feature-1 content type line 23 ObjectType-Article-1 ObjectType-Feature-2
ISSN:	0003-2670 1873-4324
DOI:	10.1016/j.aca.2007.12.045