Search Results - "Diprose, Jonathan M"
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Recent advances in the production of proteins in insect and mammalian cells for structural biology
Published in Journal of structural biology (01-10-2010)“…The production of proteins in sufficient quantity and of appropriate quality is an essential pre-requisite for structural studies. Escherichia coli remains the…”
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A procedure for setting up high-throughput nanolitre crystallization experiments. Crystallization workflow for initial screening, automated storage, imaging and optimization
Published in Acta crystallographica. Section D, Biological crystallography. (01-06-2005)“…Crystallization trials at the Division of Structural Biology in Oxford are now almost exclusively carried out using a high‐throughput workflow implemented in…”
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The atomic structure of the bluetongue virus core
Published in Nature (London) (01-10-1998)“…The structure of the core particle of bluetongue virus has been determined by X-ray crystallography at a resolution approaching 3.5 A. This transcriptionally…”
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Crystal lattice as biological phenotype for insect viruses
Published in Protein science (01-10-2005)“…Many insect viruses survive for long periods by occlusion within robust crystalline polyhedra composed primarily of a single polyhedrin protein. We show that…”
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Insights into assembly from structural analysis of bacteriophage PRD1
Published in Nature (04-11-2004)“…The structure of the membrane-containing bacteriophage PRD1 has been determined by X-ray crystallography at about 4 A resolution. Here we describe the…”
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Membrane structure and interactions with protein and DNA in bacteriophage PRD1
Published in Nature (04-11-2004)“…Membranes are essential for selectively controlling the passage of molecules in and out of cells and mediating the response of cells to their environment…”
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The Protein Information Management System (PiMS): a generic tool for any structural biology research laboratory
Published in Acta crystallographica. Section D, Biological crystallography. (01-04-2011)“…The techniques used in protein production and structural biology have been developing rapidly, but techniques for recording the laboratory information produced…”
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xtalPiMS: A PiMS-based web application for the management and monitoring of crystallization trials
Published in Journal of structural biology (01-08-2011)“…A major advance in protein structure determination has been the advent of nanolitre-scale crystallization and (in a high-throughput environment) the…”
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Recording information on protein complexes in an information management system
Published in Journal of structural biology (01-08-2011)“…The Protein Information Management System (PiMS) is a laboratory information management system (LIMS) designed for use with the production of proteins in a…”
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Benefits of Automated Crystallization Plate Tracking, Imaging, and Analysis
Published in Structure (London) (01-02-2005)“…We describe the design of a database and software for managing and organizing protein crystallization data. We also outline the considerations behind the…”
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Application of In-Fusion™ cloning for the parallel construction of E. coli expression vectors
Published in Methods in molecular biology (Clifton, N.J.) (01-01-2014)“…In-Fusion™ cloning is a flexible DNA ligase-independent cloning technology that has wide-ranging uses in molecular biology. In this chapter we describe the…”
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The Highly Ordered Double-Stranded RNA Genome of Bluetongue Virus Revealed by Crystallography
Published in Cell (14-05-1999)“…The concentration of double-stranded RNA within the bluetongue virus core renders the genome segments liquid crystalline. Powder diffraction rings confirm this…”
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