Translocation portals for the substrates and products of a viral transcription complex: the bluetongue virus core

The bluetongue virus core is a molecular machine that simultaneously and repeatedly transcribes mRNA from 10 segments of viral double‐stranded RNA, packaged in a liquid crystalline array. To determine how the logistical problems of transcription within a sealed shell are solved, core crystals were s...

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Bibliographic Details
Published in:	The EMBO journal Vol. 20; no. 24; pp. 7229 - 7239
Main Authors:	Diprose, J.M., Burroughs, J.N., Sutton, G.C., Goldsmith, A., Gouet, P., Malby, R., Overton, I., Ziéntara, S., Mertens, P.P.C., Stuart, D.I., Grimes, J.M.
Format:	Journal Article
Language:	English
Published:	Chichester, UK John Wiley & Sons, Ltd 17-12-2001 Blackwell Publishing Ltd EMBO Press Oxford University Press
Subjects:	Base Sequence Binding Sites Biochemistry, Molecular Biology blue tongue virus bluetongue virus Bluetongue virus - genetics Bluetongue virus - physiology Calcium - metabolism Crystallography Crystallography, X-Ray Crystals Life Sciences Magnesium - metabolism Phosphates - metabolism Reoviridae RNA, Messenger - genetics RNA, Messenger - metabolism Sulfates - metabolism transcription Transcription, Genetic Translocation virus structure X-ray crystallography
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Summary:	The bluetongue virus core is a molecular machine that simultaneously and repeatedly transcribes mRNA from 10 segments of viral double‐stranded RNA, packaged in a liquid crystalline array. To determine how the logistical problems of transcription within a sealed shell are solved, core crystals were soaked with various ligands and analysed by X‐ray crystallography. Mg2+ ions produce a slight expansion of the capsid around the 5‐fold axes. Oligonucleotide soaks demonstrate that the 5‐fold pore, opened up by this expansion, is the exit site for mRNA, whilst nucleotide soaks pinpoint a separate binding site that appears to be a selective channel for the entry and exit of substrates and by‐products. Finally, nucleotides also bind to the outer core layer, providing a substrate sink.
Bibliography:	ArticleID:EMBJ7594210 ark:/67375/WNG-X6M8RCR2-R istex:463BD37B7E2E8279EE4711656D2965DFE2C344A5 ObjectType-Article-2 SourceType-Scholarly Journals-1 ObjectType-Feature-1 content type line 23 ObjectType-Article-1 ObjectType-Feature-2
ISSN:	0261-4189 1460-2075 1460-2075
DOI:	10.1093/emboj/20.24.7229