Semi-interpenetrating networks of hyaluronic acid in degradable PEG hydrogels for cartilage tissue engineering

Semi-interpenetrating networks of hyaluronic acid in degradable PEG hydrogels for cartilage tissue engineering

Hydrolytically biodegradable poly(ethylene glycol) (PEG) hydrogels offer a promising platform for chondrocyte encapsulation and tuning degradation for cartilage tissue engineering, but offer no bioactive cues to encapsulated cells. This study tests the hypothesis that a semi-interpenetrating network...

Full description

Saved in:
Bibliographic Details
Published in:Acta biomaterialia Vol. 10; no. 8; pp. 3409 - 3420
Main Authors: Skaalure, Stacey C., Dimson, Shash O., Pennington, Ashley M., Bryant, Stephanie J.
Format: Journal Article
Language:English
Published: England Elsevier Ltd 01-08-2014
Subjects:
Absorbable Implants
Animals
Biocompatible Materials - chemical synthesis
Cartilage tissue engineering
Cattle
Cell Proliferation - physiology
Cell Survival - physiology
Cells, Cultured
Chondrocytes - cytology
Chondrocytes - physiology
Chondrocytes - transplantation
Chondrogenesis - physiology
Collagens
Crosslinking
Culture
Degradable biomaterials
Degradation
Encapsulation
Equipment Failure Analysis
Hyaluronic acid
Hyaluronic Acid - chemistry
Hydrogels
Hydrogels - chemistry
Hydroxyapatite
Materials Testing
Networks
Poly(ethylene glycol) hydrogel
Polyethylene Glycols - chemistry
Prosthesis Design
Tissue Engineering - instrumentation
Tissue Engineering - methods
Tissue Scaffolds
Cartilage tissue engineering
Poly(ethylene glycol) hydrogel
Hyaluronic acid
Degradable biomaterials
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:Hydrolytically biodegradable poly(ethylene glycol) (PEG) hydrogels offer a promising platform for chondrocyte encapsulation and tuning degradation for cartilage tissue engineering, but offer no bioactive cues to encapsulated cells. This study tests the hypothesis that a semi-interpenetrating network of entrapped hyaluronic acid (HA), a bioactive molecule that binds cell surface receptors on chondrocytes, and crosslinked degradable PEG improves matrix synthesis by encapsulated chondrocytes. Degradation was achieved by incorporating oligo (lactic acid) segments into the crosslinks. The effects of HA molecular weight (MW) (2.9×104 and 2×106Da) and concentration (0.5 and 5mgg−1) were investigated. Bovine chondrocytes were encapsulated in semi-interpenetrating networks and cultured for 4weeks. A steady release of HA was observed over the course of the study with 90% released by 4weeks. Incorporation of HA led to significantly higher cell numbers throughout the culture period. After 8days, HA increased collagen content per cell, increased aggrecan-positive cells, while decreasing the deposition of hypertrophic collagen X, but these effects were not sustained long term. Measuring total sulfated glycosaminoglycan (sGAG) and collagen content within the constructs and released to the culture medium after 4weeks revealed that total matrix synthesis was elevated by high concentrations of HA, indicating that HA stimulated matrix production although this matrix was not retained within the hydrogels. Matrix-degrading enzymes were elevated in the low-, but not the high-MW HA. Overall, incorporating high-MW HA into degrading hydrogels increased chondrocyte number and sGAG and collagen production, warranting further investigations to improve retention of newly synthesized matrix molecules.
Bibliography:ObjectType-Article-1
SourceType-Scholarly Journals-1
ObjectType-Feature-2
content type line 23
ISSN:1742-7061
1878-7568
DOI:10.1016/j.actbio.2014.04.013