Search Results - "Dimond, Randall L."

Functional heterogeneity of monoclonal antibodies obtained using different screening assays by Mierendorf, Jr, R C, Dimond, R L

“…Two alternate screening methods have enabled the detection of monoclonal antibodies with different specificities toward the lysosomal enzyme alpha-mannosidase…”
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The use of tolerization in the production of monoclonal antibodies against minor antigenic determinants by Golumbeski, Jr, G S, Dimond, R L

“…An initial attempt to prepare monoclonal antibodies specific for the Dictyostelium discoideum lysosomal enzyme beta-glucosidase was unsuccessful. All of the…”
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Regulation of protein synthesis in Dictyostelium discoideum: effects of starvation and anoxia on initiation by Cardelli, James A, Dimond, Randall L

“…A rapid reduction in the size and amount of total polysomes is one of the earliest observed changes when the cellular slime mold Dictyostelium discoideum is…”
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Isolation and characterization of mutations affecting UDPG pyrophosphorylase activity in Dictyostelium discoideum by Dimond, Randall L., Farnsworth, Paul A., Loomis, William F.

“…A procedure for screening large numbers of clones for an enzyme activity was used to isolate mutations which affect UDPG pyrophosphorylase activity (EC…”
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Mutations Affecting N-Acetylglucosaminidase in Dictyostelium discoideum by Dimond, Randall L., Brenner, Michael, Loomis, William F.

“…A procedure for screening several thousand clones for alterations in a specific gene product was apapplied to mutagenized cultures of Dictyostelium discoideum…”
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Vegetative Isozyme of N-Acetylglucosaminidase in Dictyostelium discoideum by Dimond, R L, Loomis, Jr, W F

“…A minor isozyme of N -acetylglucosaminidase was isolated from growing cells of Dictyostelium discoideum and purified more than 1000-fold. The minor isozyme…”
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Visualization of antigenic proteins on Western blots by Knecht, D A, Dimond, R L

“…A new technique for the detection of antibodies bound to proteins blotted onto nitrocellulose paper was developed. The method is rapid, sensitive, and does not…”
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Pathways Involved in Targeting and Secretion of a Lysosomal Enzyme in Dictyostelium discoideum by Mierendorf, Robert C., Cardelli, James A., Dimond, Randall L.

“…In Dictyostelium discoideum, the lysosomal enzyme α-mannosidase is first synthesized as an N-glycosylated precursor of Mr 140,000. After a 20-30-min lag…”
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Inhibition of Early but Not Late Proteolytic Processing Events Leads to the Missorting and Oversecretion of Precursor Forms of Lysosomal Enzymes in Dictyostelium discoideum by Richardson, Jan M., Woychik, Nancy A., Ebert, David L., Dimond, Randall L., Cardelli, James A.

“…Lysosomal enzymes are initially synthesized as precursor polypeptides which are proteolytically cleaved to generate mature forms of the enzymatically active…”
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The α-glucosidases of Dictyostelium discoideum by Borts, Rhona H., Dimond, Randall L.

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The α-glucosidases of Dictyostelium discoideum by Borts, Rhona H., Dimond, Randall L.

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Lysosomal Enzymes in Dictyostelium discoideum Are Transported to Lysosomes at Distinctly Different Rates by Cardelli, James A., Golumbeski, George S., Dimond, Randall L.

“…We are investigating the molecular mechanisms involved in the localization of lysosomal enzymes in Dictyostelium discoideum, an organism that lacks any…”
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Lysosomal enzyme secretory mutants of Dictyostelium discoideum by Ebert, D.L, Jordan, K.B, Dimond, R.L

“…Dictyostelium discoideum secretes a number of lysosomal enzymes during axenic growth and upon suspension in a low ionic strength, non-nutrient buffer (standard…”
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A Dictyostelium discoideum Mutant That Missorts and Oversecretes Lysosomal Enzyme Precursors Is Defective in Endocytosis by Ebert, David L., Freeze, Hudson H., Richardson, Jan, Dimond, Randall L., Cardelli, James A.

“…A mutant strain of Dictyostelium discoideum, HMW570, oversecretes several lysosomal enzyme activities during growth. Using a radiolable pulse-chase protocol,…”
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Membrane-bound and free polysomes in Dictyostelium discoideum by Cardelli, James A., Knecht, David A., Dimond, Randall L.

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Major changes in gene expression occur during at least four stages of development of Dictyostelium discoideum by Cardelli, James A., Knecht, David A., Wunderlich, Roxanne, Dimond, Randall L.

“…The spectrum of proteins synthesized at different stages of development of the cellular slime mold Dictyostelium discoideum was analyzed by two-dimensional…”
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Biogenesis of lysosomal enzymes in the alpha-glucosidase II-deficient modA mutant of Dictyostelium discoideum: retention of alpha-1,3-linked glucose on N-linked oligosaccharides delays intracellular transport but does not alter sorting of alpha-mannosidase or beta-glucosidase by Ebert, D L, Bush, J M, Dimond, R L, Cardelli, J A

“…The endoplasmic reticulum-localized enzyme alpha-glucosidase II is responsible for removing the two alpha-1,3-linked glucose residues from N-linked…”
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Maturation of asparagine-linked oligosaccharides in Dictyostelium discoideum analyzed with modification-specific probes by Judelson, H S, Dimond, R L

“…Lysosomal enzymes in Dictyostelium discoideum contain high mannose oligosaccharides that contain mannose 6-phosphate and several unusual structures. The…”
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Characterization and distribution of multiple antigens on N-linked oligosaccharides of Dictyostelium discoideum proteins by Judelson, H S, Freeze, H H, Dimond, R L

“…Monoclonal antibodies were prepared against a mixture of purified lysosomal enzymes from Dictyostelium discoideum. Three classes of antibodies were found which…”
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The α-glucosidases of Dictyostelium discoideum: II. Developmental regulation and cellular localization by Borts, Rhona H., Dimond, Randall L.

“…Four isozymes of α-glucosidase in Dictyostelium discoideum have been identified and some of their enzymatic and physical properties characterized (R. H. Borts…”
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