Molecular diagnosis of CMV infection in fetal aborted tissues in the region of Thrace

Molecular diagnosis of CMV infection in fetal aborted tissues in the region of Thrace

To detect the incidence of CMV infection in spontaneous abortion in Thrace. Genetic material from 143 fetuses aged from 11 to 39 weeks was examined. The material originated from various regions of Thrace. All fetuses and the respective placentas underwent routine histopathology. DNA was isolated fro...

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Published in:Clinical and experimental obstetrics & gynecology Vol. 39; no. 1; pp. 96 - 102
Main Authors: Grammatikopoulou, I, Lambropoulou, M, Chatzaki, E, Deftereou, T E, Lambropoulou, V, Simopoulou, M, Papadopoulos, E, Galazios, G, Dimitriou, Th, Petrou, A, Papadopoulos, N
Format: Journal Article
Language:English
Published: Canada 2012
Subjects:
Aborted Fetus - pathology
Abortion, Spontaneous - pathology
Abortion, Spontaneous - virology
Adolescent
Adult
Cytomegalovirus - isolation & purification
Cytomegalovirus Infections - complications
Female
Greece
Humans
Male
Polymerase Chain Reaction
Pregnancy
Pregnancy Complications, Infectious - virology
Young Adult
Greece
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Summary:To detect the incidence of CMV infection in spontaneous abortion in Thrace. Genetic material from 143 fetuses aged from 11 to 39 weeks was examined. The material originated from various regions of Thrace. All fetuses and the respective placentas underwent routine histopathology. DNA was isolated from sections of paraffinized tissues. Detection of CMV in the DNA genomic samples was performed using a commercial PCR-based detection kit. From the 143 fetuses that were examined, two were found to be CMV positive. Pathological findings related to inflammatory corruptions were observed in the placentas of 97 embryos, including the CMV infected ones. This study indicates CMV-DNA infection in 1.4% of aborted fetuses. CMV infection incidence in aborted fetuses is similar to this reported in other European regions. The molecular technique of PCR applied on paraffin-embedded biopsy material is proven to be an accurate, valid and fast method for investigating the CMV infection in aborted fetuses.
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ISSN:0390-6663