Preparative separation of naphthyltetrahydroisoquinoline alkaloids from Ancistrocladus korupensis by centrifugal partition chromatography

Crude extracts of Ancistrocladus korupensis contain a complex mixture of naphthyltetrahydroisoquinoline alkaloids, including the human immunodeficiency virus-inhibitory dimeric alkaloids michellamines A and B and the antimalarial monomeric korupensamines A-D. The efficient separation of michellamine...

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Bibliographic Details
Published in:	Journal of chromatography. A Vol. 688; no. 1-2; p. 83
Main Authors:	Hallock, Y F, Dai, J, Bokesch, H R, Dillah, K B, Manfredi, K P, Cardellina, 2nd, J H, Boyd, M R
Format:	Journal Article
Language:	English
Published:	Netherlands 30-12-1994
Subjects:	Alkaloids - isolation & purification Antiviral Agents - isolation & purification Chromatography, High Pressure Liquid - methods Isoquinolines - isolation & purification Naphthalenes - isolation & purification Plants, Medicinal - chemistry Spectrophotometry, Ultraviolet
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Summary:	Crude extracts of Ancistrocladus korupensis contain a complex mixture of naphthyltetrahydroisoquinoline alkaloids, including the human immunodeficiency virus-inhibitory dimeric alkaloids michellamines A and B and the antimalarial monomeric korupensamines A-D. The efficient separation of michellamines A and B from these extracts has been accomplished by centrifugal partition chromatography. The chromatographic conditions used on a multi-channel cartridge unit (Sanki LLN) have been successfully scaled up with a newly developed, stacked-disk type centrifugal partition chromatography unit (Sanki NMF) for separating larger amounts of alkaloid mixtures with similar resolution. A refined, three-step process (solvent-solvent partitioning, centrifugal partition chromatography and HPLC) has been developed and applied to the scaled-up production of michellamine B for preclinical drug development.
ISSN:	0021-9673
DOI:	10.1016/0021-9673(94)00923-6