Search Results - "Diers, I"
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Yield improvement of heterologous peptides expressed in yps1-disrupted Saccharomyces cerevisiae strains
Published in Enzyme and microbial technology (01-06-2000)“…Heterologous protein expression levels in Saccharomyces cerevisiae fermentations are highly dependent on the susceptibility to endogenous yeast proteases…”
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Nucleotide sequence of a beta-1,3-glucanase isoenzyme IIA gene of Oerskovia xanthineolytica LL G109 (Cellulomonas cellulans) and initial characterization of the recombinant enzyme expressed in Bacillus subtilis
Published in Journal of Bacteriology (01-08-1996)“…Article Usage Stats Services JB Citing Articles Google Scholar PubMed Related Content Social Bookmarking CiteULike Delicious Digg Facebook Google+ Mendeley…”
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Molecular cloning of a lytic beta-1,3-glucanase gene from Oerskovia xanthineolytica LLG109. A beta-1,3-glucanase able to selectively permeabilize the yeast cell wall
Published in Annals of the New York Academy of Sciences (15-05-1996)“…Molecular cloning of the beta gIII gene encoding for an endo-beta-1,3-glucanase (beta gl II) from Oerskovia xanthineolytica LLG109, a yeast-lytic gram-positive…”
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Engineering-enhanced Protein Secretory Expression in Yeast with Application to Insulin
Published in The Journal of biological chemistry (24-05-2002)“…Adaptation to efficient heterologous expression is a prerequisite for recombinant proteins to fulfill their clinical and biotechnological potential. We…”
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Purification and characterisation of a new hypothalamic satiety peptide, cocaine and amphetamine regulated transcript (CART), produced in yeast
Published in FEBS letters (29-05-1998)“…Cocaine and amphetamine regulated transcript (CART) is a newly discovered hypothalamic peptide with a potent appetite suppressing activity following…”
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Yeast fermentation processes for insulin production
Published in Bioprocess technology (1991)Get more information
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Aprotinin and aprotinin analogues expressed in yeast
Published in Biological chemistry Hoppe-Seyler (01-05-1990)“…Synthetic genes encoding aprotinin and aprotinin analogues were constructed and fused in frame to the S. cerevisiae mating factor alpha 1 signal-leader (1-85)…”
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Expression of Insulin in Yeast: The Importance of Molecular Adaptation for Secretion and Conversion
Published in Biotechnology & genetic engineering reviews (01-07-2001)Get full text
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Yeast cell permeabilizing β-1,3-Glucanases: A tool for the integration of downstream processes and metabolic engineering applications to yeast
Published in Biotechnology and bioengineering (20-04-1998)“…In this article, we consider the impact on downstream process design resulting from the use of metabolically engineered yeast strains. We address the issue of…”
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Journal Article -
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Nucleotide sequence of a beta -1,3-glucanase isoenzyme II sub(A) gene of Oerskovia xanthineolytica LL G109 (Cellulomonas cellulans) and initial characterization of the recombinant enzyme expressed in Bacillus subtilis
Published in Journal of bacteriology (01-01-1996)“…The nucleotide sequence of the beta glII sub(A) gene, encoding the extracellular beta -1,3-glucanase II sub(A) ( beta glII sub(A)) of the yeast-lytic…”
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Synthetic leaders with potential BiP binding mediate high-yield secretion of correctly folded insulin precursors from Saccharomyces cerevisiae
Published in Protein expression and purification (01-04-1997)“…Secretion leaders are essential for expression of many heterologous proteins including insulin in yeast. The function of secretion leaders and their…”
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Alpha‐Factor Pro‐Peptide N‐linked Oligosaccharides Facilitate Secretion of the Insulin Precursor in Saccharomyces Cerevisiae
Published in Biotechnology and applied biochemistry (01-04-1998)“…Toevaluate the possible relationship between N‐linked glycosylation ofthe Saccharomycescerevisiae a‐factor pro‐peptideand transport of the a‐factor…”
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Soluble, prolonged-acting insulin derivatives. II. Degree of protraction and crystallizability of insulins substituted in positions A17, B8, B13, B27 and B30
Published in Protein engineering (01-06-1987)“…It has previously been found that insulins, to which positive charge has been added by substitutions in position B30, thus raising the isoelectric point…”
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Soluble, prolonged-acting insulin derivatives. III. Degree of protraction, crystallizability and chemical stability of insulins substituted in positions A21, B13, B23, B27 and B30
Published in Protein engineering (01-07-1988)“…It was previously demonstrated that insulins to which positive charge has been added by substituting B13 glutamic acid with a glutamine residue, B27 threonine…”
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