Toxoplasma membrane inositol phospholipid binding protein TgREMIND is essential for secretory organelle function and host infection

Apicomplexan parasites possess specialized secretory organelles called rhoptries, micronemes, and dense granules that play a vital role in host infection. In this study, we demonstrate that TgREMIND, a protein found in Toxoplasma gondii, is necessary for the biogenesis of rhoptries and dense granule...

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Published in:	Cell reports (Cambridge) Vol. 43; no. 1; p. 113601
Main Authors:	Houngue, Rodrigue, Sangaré, Lamba Omar, Alayi, Tchilabalo Dilezitoko, Dieng, Aissatou, Bitard-Feildel, Tristan, Boulogne, Claire, Slomianny, Christian, Atindehou, Cynthia Menonve, Fanou, Lucie Ayi, Hathout, Yetrib, Callebaut, Isabelle, Tomavo, Stanislas
Format:	Journal Article
Language:	English
Published:	United States Elsevier Inc 23-01-2024 Elsevier
Subjects:	apicomplexan parasites CP: Microbiology F-BAR-containing protein host cell infection Life Sciences lineage-specific organelles membrane lipid binding Microbiology and Parasitology Parasitology REMIND domain TgREMIND protein Toxoplasma gondii Toxoplasma gondii membrane lipid binding TgREMIND protein REMIND domain F-BAR-containing protein CP: Microbiology apicomplexan parasites lineage-specific organelles host cell infection
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Summary:	Apicomplexan parasites possess specialized secretory organelles called rhoptries, micronemes, and dense granules that play a vital role in host infection. In this study, we demonstrate that TgREMIND, a protein found in Toxoplasma gondii, is necessary for the biogenesis of rhoptries and dense granules. TgREMIND contains a Fes-CIP4 homology-Bin/Amphiphysin/Rvs (F-BAR) domain, which binds to membrane phospholipids, as well as a novel uncharacterized domain that we have named REMIND (regulator of membrane-interacting domain). Both the F-BAR domain and the REMIND are crucial for TgREMIND functions. When TgREMIND is depleted, there is a significant decrease in the abundance of dense granules and abnormal transparency of rhoptries, leading to a reduction in protein secretion from these organelles. The absence of TgREMIND inhibits host invasion and parasite dissemination, demonstrating that TgREMIND is essential for the proper function of critical secretory organelles required for successful infection by Toxoplasma. [Display omitted] •TgREMIND is a membrane-associated protein binding to PIP2 lipids•Both F-BAR domain and REMIND of TgREMIND are required for PIP2 binding•TgREMIND is essential for the biogenesis of rhoptries and dense granules in Toxoplasma•Inactivation of TgREMIND abolished Toxoplasma host cell invasion and dissemination In this study, Houngue et al. describe an essential role for the uncharacterized protein named TgREMIND, for regulator of membrane-interacting domain, in the biogenesis of rhoptries and dense granules, two secretory organelles that contain virulence factors released during and after Toxoplasma cell invasion.
Bibliography:	ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 23
ISSN:	2211-1247 2211-1247
DOI:	10.1016/j.celrep.2023.113601