CHARACTERISTICS AND OPTIMAL WORKING CONDITIONS OF AMPEROMETRIC BIOSENSOR FOR ADENOSINE TRIPHOSPHATE DETERMINATION
Analytical characteristics of a biosensor based on glucose oxidase and hexokinase and intended for ATP determination were studied. Platinum disc electrodes were used as amperometric transducers. Range of working potentials for biosensor functioning was shown. An optimal time of enzymes immobilizatio...
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| Published in: | Biotechnologia acta Vol. 7; no. 1; pp. 66 - 74 |
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| Main Authors: | , , , |
| Format: | Journal Article |
| Language: | English |
| Published: |
Kyiv
Palladin Institute of Biochemistry of National Academy of Sciences of Ukraine
01-01-2014
National Academy of Sciences of Ukraine, Palladin Institute of Biochemistry |
| Subjects: | |
| Online Access: | Get full text |
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| Summary: | Analytical characteristics of a biosensor based on glucose oxidase and hexokinase and intended for ATP determination were studied. Platinum disc electrodes were used as amperometric transducers. Range of working potentials for biosensor functioning was shown. An optimal time of enzymes immobilization was determined. Optimal conditions for biosensor functioning during work with biological fluids were selected. Biosensor work in three buffer solutions (PBS, tris and HEPES) was investigated and it was shown that it was possible to obtain various operational characteristics of the biosensor depending on tasks that are assigned to it by varying the composition of sample. Reproducibility of biosensor responses to ATP and glucose during a day and of biosensor preparation was shown. The proposed biosensor can be further used for analysis of glucose and ATP content in water solutions. |
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| Bibliography: | ObjectType-Article-2 SourceType-Scholarly Journals-1 ObjectType-Feature-1 content type line 23 |
| ISSN: | 2410-7751 1995-5537 2410-776X |
| DOI: | 10.15407/biotech7.01.066 |