Ethylenediamine grafted carbon nanotube aerogels modified screen-printed electrode for simultaneous electrochemical immunoassay of multiple tumor markers

Ethylenediamine grafted carbon nanotube aerogels modified screen-printed electrode for simultaneous electrochemical immunoassay of multiple tumor markers

•Hybrid carbon aerogels were synthesized and introduced for immunosensor application.•The sandwich configuration immunosensor was based on the screen-printed technology.•Redox mediators (dyes) have been used as labels.•The improved immunosensor was employed for individual and simultaneous electroche...

Full description

Saved in:
Bibliographic Details
Published in:Journal of electroanalytical chemistry (Lausanne, Switzerland) Vol. 900; p. 115700
Main Authors: Filik, Hayati, Avan, Asiye Aslıhan, Altaş Puntar, Nilay, Özyürek, Mustafa, Güngör, Zeynep Banu, Kucur, Mine, Kamış, Handan, Dicle, Diclehan Ali
Format: Journal Article
Language:English
Published: Amsterdam Elsevier B.V 01-11-2021
Elsevier Science Ltd
Subjects:
Aerogels
Alpha-fetoprotein
Antibodies
Antigens
Carcinoembryonic antigen
Electrochemistry
Ethylenediamine
Fourier transforms
Immunoassay
Immunosensor
Immunosensors
Infrared analysis
Multi wall carbon nanotubes
Safranine
Sandwich structures
Serum analysis
Square waves
Tumors
Voltammetry
Electrochemistry
Carcinoembryonic antigen
Serum analysis
Immunosensor
Alpha-fetoprotein
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:•Hybrid carbon aerogels were synthesized and introduced for immunosensor application.•The sandwich configuration immunosensor was based on the screen-printed technology.•Redox mediators (dyes) have been used as labels.•The improved immunosensor was employed for individual and simultaneous electrochemical detection of AFP and CEA.•The developed EC sensor provided a sufficient linear range of 0.005–1.0 ng/mL for AFP and CEA. A sensitive sandwich-model immunoassay designed for simultaneous electrochemical measurement of alpha-fetoprotein and carcinoembryonic antigen handling multiple-label strategy. The sandwich-model immunosensor was constructed by assembling ethylenediamine-MWCNT aerogels (EDA-CAGs), AuNPs and capture antibodies (Cp-Ab1) on the screen-printed carbon electrode (SPCE). The prepared EDA-CAG textures were analyzed using Fourier transform infrared (FT-IR), Scanning Electron Microscopy (SEM) and Energy Dispersive X-Ray Spectroscopy (EDX). In this protocol, AuNPs decorated EDA-CAG-carried Thionine (Thi) and AuNPs decorated EDA-CAG-carried Safranine O (SfO) (denoted as AuNP-Thi-EDA-CAG and AuNP-SfO-EDA-CAG, respectively) were properly exploited as distinguishable signal labels, which were used to attach detection antibodies (Ab2) (anti-AFP2 and anti-CEA2), respectively. When two tumor antigens were present, a square wave voltammetry (SWV) scan displayed two well-separated signals, each signal indicated one target antigen. Observed results introduced that the square wave voltammetric peak current exhibited a good linear relationship to logarithm concentration in the ranges from 0.005 to 1.0 ng/mL for both analytes. The LoDs were 0.0015 and 0.0010 ng/mL (at signal/noise S/N = 3) for AFP and CEA, respectively. The designed sandwich-type immunosensor was applied to real serum sample analysis.
ISSN:1572-6657
1873-2569
DOI:10.1016/j.jelechem.2021.115700