Validation of Gene Expression Patterns for Oral Feeding Readiness: Transcriptional Analysis of Set of Genes in Neonatal Salivary Samples

Validation of Gene Expression Patterns for Oral Feeding Readiness: Transcriptional Analysis of Set of Genes in Neonatal Salivary Samples

Neonatal health assessment is crucial for detecting and intervening in various disorders. Traditional gene expression analysis methods often require invasive procedures during sample collection, which may not be feasible or ideal for preterm infants. In recent years, saliva has emerged as a promisin...

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Bibliographic Details
Published in:Genes Vol. 15; no. 7; p. 936
Main Authors: Gomes, Leonardo Henrique Ferreira, Marques, Andressa Brito, Dias, Isabel Cristina de Meireles, Gabeira, Sanny Cerqueira de O, Barcelos, Tamara Rosa, Guimarães, Mariana de Oliveira, Ferreira, Igor Ribeiro, Guida, Letícia Cunha, Lucena, Sabrina Lopes, Rocha, Adriana Duarte
Format: Journal Article
Language:English
Published: Switzerland MDPI AG 18-07-2024
Subjects:
Analysis
Biomarkers
Clinical medicine
Enteral nutrition
Feeding
Foxp2 protein
Gene expression
Genes
Genetic aspects
Genetic engineering
Genetic transcription
Gestational age
Infants
Infants (Premature)
Kinases
Neonates
Neonatology
Physiology
Premature babies
Saliva
Scientific equipment and supplies industry
Success
Transcriptomics
United States
United States > US
Germany
neonatal
oral feeding
salivary samples
gene expression
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Summary:Neonatal health assessment is crucial for detecting and intervening in various disorders. Traditional gene expression analysis methods often require invasive procedures during sample collection, which may not be feasible or ideal for preterm infants. In recent years, saliva has emerged as a promising noninvasive biofluid for assessing gene expression. Another trend that has been growing is the use of " " technologies such as transcriptomics in the analysis of gene expression. The costs for carrying out these analyses and the difficulty of analysis make the detection of candidate genes necessary. These genes act as biomarkers for the maturation stages of the oral feeding issue. Salivary samples (n = 225) were prospectively collected from 45 preterm (<34 gestational age) infants from five predefined feeding stages and submitted to RT-qPCR. A better description of the targeted genes and results from RT-qPCR analyses were included. The six genes previously identified as predictive of feeding success were tested. The genes are , , , , , and , along with two reference genes: and . RT-qPCR amplification enabled the analysis of the gene expression of , , , , , and in neonatal saliva. Expression results were correlated with the feeding status during sample collection. In summary, the genes , , , , , and play critical roles in regulating oral feeding and the development of premature infants. Understanding the influence of these genes can provide valuable insights for improving nutritional care and support the development of these vulnerable babies. Evidence suggests that saliva-based gene expression analysis in newborns holds great promise for early detection and monitoring of disease and understanding developmental processes. More research and standardization of protocols are needed to fully explore the potential of saliva as a noninvasive biomarker in neonatal care.
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ISSN:2073-4425
2073-4425
DOI:10.3390/genes15070936