Enzymatic studies of cisplatin induced oxidative stress in hepatic tissue of rats

Enzymatic studies of cisplatin induced oxidative stress in hepatic tissue of rats

Cisplatin is an active cytotoxic agent that has proved to be successful in the treatment of various types of solid tumors. The drug-induced nephrotoxicity has been very well documented in clinical oncology. However, hepatotoxicity has been rarely characterized and paid attention to, and is the least...

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Bibliographic Details
Published in:European journal of pharmacology Vol. 532; no. 3; pp. 290 - 293
Main Authors: Pratibha, R., Sameer, R., Rataboli, Padmanabh V., Bhiwgade, Dayanand A., Dhume, Chitra Y.
Format: Journal Article
Language:English
Published: Amsterdam Elsevier B.V 27-02-2006
Elsevier
Subjects:
Animals
Antineoplastic Agents - toxicity
Biological and medical sciences
Body Weight - drug effects
Cisplatin
Cisplatin - toxicity
Cytochrome P-450 Enzyme System - analysis
Cytochrome P-450 Enzyme System - metabolism
Cytochromes b5 - analysis
Cytochromes b5 - metabolism
Enzymatic antioxidant
Glutathione Reductase - metabolism
Hepatotoxicity
Lipid peroxidation
Lipid Peroxidation - drug effects
Liver - drug effects
Liver - enzymology
Male
Medical sciences
Organ Size - drug effects
Oxidative Stress
Pharmacology. Drug treatments
Rats
Rats, Wistar
Lipid peroxidation
Enzymatic antioxidant
Hepatotoxicity
Cisplatin
Antineoplastic agent
Oxidative stress
Rat
Digestive system
Liver
Rodentia
Alkylating agent
Vertebrata
Mammalia
Animal
Platinum II Complexes
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Summary:Cisplatin is an active cytotoxic agent that has proved to be successful in the treatment of various types of solid tumors. The drug-induced nephrotoxicity has been very well documented in clinical oncology. However, hepatotoxicity has been rarely characterized and paid attention to, and is the least studied. We have used rat as the model to evaluate the effect of cisplatin on liver antioxidant enzymes and to determine whether these modulations in enzymatic activities are involved in hepatotoxicity. Reports obtained from our study indicate that cisplatin increases lipid peroxidation in the treated tissue of rat. The drug is also involved in altering the thiol status of the tissue with concomitant alterations in the enzymatic antioxidants. Glutathione and glutathione reductase levels were significantly decreased after cisplatin therapy, whereas glutathione peroxidase, gamma-glutamyl transpeptidase and catalase showed a significant increase. No statistically significant change was observed in glutathione- S-transferase activity. After cisplatin treatment, cytochrome P 450 showed a significant increase, whereas cytochrome b 5 was decreased. Thus, an alteration in enzymatic antioxidant status with increase in lipid peroxidation indicates that the enzymes play an important role in combating free radical induced oxidative stress on the tissue.
Bibliography:ObjectType-Article-1
SourceType-Scholarly Journals-1
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content type line 23
ISSN:0014-2999
1879-0712
DOI:10.1016/j.ejphar.2006.01.007