Antibacterial and Antioxidant Activities of Novel Actinobacteria Strain Isolated from Gulf of Khambhat, Gujarat
Bacterial secondary metabolites possess a wide range of biologically active compounds including antibacterial and antioxidants. In this study, a Gram-positive novel marine was isolated from sea sediment which showed 84% 16S rRNA gene sequence (KT588655) similarity with (EU841661) and designated as R...
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| Published in: | Frontiers in microbiology Vol. 8; p. 2420 |
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| Main Authors: | , , , , |
| Format: | Journal Article |
| Language: | English |
| Published: |
Switzerland
Frontiers Media S.A
07-12-2017
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| Subjects: | |
| Online Access: | Get full text |
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| Summary: | Bacterial secondary metabolites possess a wide range of biologically active compounds including antibacterial and antioxidants. In this study, a Gram-positive novel marine
was isolated from sea sediment which showed 84% 16S rRNA gene sequence (KT588655) similarity with
(EU841661) and designated as
RD-5. The genus
is considered as a promising source of bioactive secondary metabolites. The isolated novel bacterial strain was characterized by antibacterial characteristics and antioxidant activities. The BIOLOG based analysis suggested that
RD-5 utilized a wide range of substrates compared to the reference strain. The result is further supported by statistical analysis such as AWCD (average well color development), heat-map and PCA (principal component analysis). The whole cell fatty acid profiling showed the dominance of iso/anteiso branched C15-C17 long chain fatty acids. The identified strain
RD-5 exhibited a broad spectrum of antibacterial activities for the Gram-negative bacteria (
NCIM 2065,
NCIM,
sp. NCIM 2200 and
NCIM), and Gram-positive bacteria (
NCIM 2920 and
MTCC 96). Extract of
strain RD-5 showed 82.86 and 89% of 2,2-diphenyl-1-picrylhydrazyl (DPPH) free radical scavenging and metal chelating activity, respectively, at 5.0 mg/mL. While H
O
scavenging activity was 74.5% at 0.05 mg/mL concentration. Furthermore, polyketide synthases (PKSs types I and II), an enzyme complex that produces polyketides, the encoding gene(s) detected in the strain RD-5 which may probably involve for the synthesis of antibacterial compound(s). In conclusion, a novel bacterial strain of
, isolated from the unexplored sea sediment of Alang, Gulf of Khambhat (Gujarat), India showed promising antibacterial activities. However, fractionation and further characterization of active compounds from
RD-5 are needed for their optimum utilization toward antibacterial purposes. |
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| Bibliography: | ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 23 Reviewed by: Osmar Nascimento Silva, Universidade Católica Dom Bosco, Brazil; Atte Von Wright, University of Eastern Finland, Finland Edited by: Jem Stach, Newcastle University, United Kingdom This article was submitted to Antimicrobials, Resistance and Chemotherapy, a section of the journal Frontiers in Microbiology |
| ISSN: | 1664-302X 1664-302X |
| DOI: | 10.3389/fmicb.2017.02420 |