Eye and hair color prediction of human DNA recovered from Lucilia sericata larvae

Eye and hair color prediction of human DNA recovered from Lucilia sericata larvae

Forensic entomological evidence is employed to estimate minimum postmortem interval (PMImin), location, and identification of fly samples or human remains. Traditional forensic DNA analysis (i.e., STR, mitochondrial DNA) has been used for human identification from the larval gut contents. Forensic D...

Full description

Saved in:
Bibliographic Details
Published in:International journal of legal medicine Vol. 138; no. 2; pp. 627 - 637
Main Authors: Deymenci, Emre, Sarı O, Ilksen, Filoglu, Gonul, Polat, Erdal, Bulbul, Ozlem
Format: Journal Article
Language:English
Published: Berlin/Heidelberg Springer Berlin Heidelberg 01-03-2024
Springer Nature B.V
Subjects:
Color
Deoxyribonucleic acid
DNA
Entomology
Eyes & eyesight
Forensic Medicine
Forensic sciences
Genetics
Hair
Larvae
Medical Law
Medicine
Medicine & Public Health
Mitochondrial DNA
Original Article
HIrisPlex
Larvae gut contents
Phenotype prediction
SNP
Lucilia sericata
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:Forensic entomological evidence is employed to estimate minimum postmortem interval (PMImin), location, and identification of fly samples or human remains. Traditional forensic DNA analysis (i.e., STR, mitochondrial DNA) has been used for human identification from the larval gut contents. Forensic DNA phenotyping (FDP), predicting human appearance from DNA-based crime scene evidence, has become an established approach in forensic genetics in the past years. In this study, we aimed to recover human DNA from Lucilia sericata (Meigen 1826) (Diptera: Calliphoridae) gut contents and predict the eye and hair color of individuals using the HIrisPlex system. Lucilia sericata larvae and reference blood samples were collected from 30 human volunteers who were under maggot debridement therapy. The human DNA was extracted from the crop contents and quantified. HIrisPlex multiplex analysis was performed using the SNaPshot minisequencing procedure. The HIrisPlex online tool was used to assess the prediction of the eye and hair color of the larval and reference samples. We successfully genotyped 25 out of 30 larval samples, and the most SNP genotypes (87.13%) matched those of reference samples, though some alleles were dropped out, producing partial profiles. The prediction of the eye colors was accurate in 17 out of 25 larval samples, and only one sample was misclassified. Fourteen out of 25 larval samples were correctly predicted for hair color, and eight were misclassified. This study shows that SNP analysis of L. sericata gut contents can be used to predict eye and hair color of a corpse.
Bibliography:ObjectType-Article-1
SourceType-Scholarly Journals-1
ObjectType-Feature-2
content type line 23
ISSN:0937-9827
1437-1596
DOI:10.1007/s00414-023-03112-z