Search Results - "Devenish, Sean R.A."

Protocol to determine antibody affinity and concentration in complex solutions using microfluidic antibody affinity profiling by Emmenegger, Marc, Worth, Roland, Fiedler, Sebastian, Devenish, Sean R.A., Knowles, Tuomas P.J., Aguzzi, Adriano

“…Conventional methods of measuring affinity are limited by artificial immobilization, large sample volumes, and homogeneous solutions. This protocol describes…”
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Both COVID-19 infection and vaccination induce high-affinity cross-clade responses to SARS-CoV-2 variants by Emmenegger, Marc, Fiedler, Sebastian, Brugger, Silvio D., Devenish, Sean R.A., Morgunov, Alexey S., Ilsley, Alison, Ricci, Francesco, Malik, Anisa Y., Scheier, Thomas, Batkitar, Leyla, Madrigal, Lidia, Rossi, Marco, Meisl, Georg, Lynn, Andrew K., Saleh, Lanja, von Eckardstein, Arnold, Knowles, Tuomas P.J., Aguzzi, Adriano

“…The B.1.1.529 (omicron) variant has rapidly supplanted most other SARS-CoV-2 variants. Using microfluidics-based antibody affinity profiling (MAAP), we have…”
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A tetrameric structure is not essential for activity in dihydrodipicolinate synthase (DHDPS) from Mycobacterium tuberculosis by Evans, Genevieve, Schuldt, Linda, Griffin, Michael D.W., Devenish, Sean R.A., Grant Pearce, F., Perugini, Matthew A., Dobson, Renwick C.J., Jameson, Geoffrey B., Weiss, Manfred S., Gerrard, Juliet A.

“…► A dimeric variant of DHDPS from Mycobacterium tuberculosis was engineered. ► The tertiary fold was preserved, but the quaternary structure was fully…”
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LC–MS and NMR characterization of the purple chromophore formed in the o-aminobenzaldehyde assay of dihydrodipicolinate synthase by Mitsakos, Voula, Devenish, Sean R.A., O’Donnell, Paul A., Gerrard, Juliet A., Hutton, Craig A.

“…The enzyme dihydrodipicolinate synthase (DHDPS) has been widely investigated as a target for new antibiotics. The o-aminobenzaldehyde (o-ABA) assay is…”
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The C-terminal domain of Escherichia coli dihydrodipicolinate synthase (DHDPS) is essential for maintenance of quaternary structure and efficient catalysis by Guo, Belinda B.B., Devenish, Sean R.A., Dobson, Renwick C.J., Muscroft-Taylor, Andrew C., Gerrard, Juliet A.

“…Dihydrodipicolinate synthase (DHDPS) catalyses the first committed step in the biosynthesis of (S)-lysine, an essential constituent of bacterial cell walls…”
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The current landscape in Alzheimer’s disease research and drug discovery by Devenish, Sean R.A.

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Kinetic fingerprints differentiate the mechanisms of action of anti-Aβ antibodies by Linse, Sara, Scheidt, Tom, Bernfur, Katja, Vendruscolo, Michele, Dobson, Christopher M., Cohen, Samuel I. A., Sileikis, Eimantas, Lundqvist, Martin, Qian, Fang, O’Malley, Tiernan, Bussiere, Thierry, Weinreb, Paul H., Xu, Catherine K., Meisl, Georg, Devenish, Sean R. A., Knowles, Tuomas P. J., Hansson, Oskar

“…The amyloid cascade hypothesis, according to which the self-assembly of amyloid-β peptide (Aβ) is a causative process in Alzheimer’s disease, has driven many…”
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One in a Million: Flow Cytometric Sorting of Single Cell-Lysate Assays in Monodisperse Picolitre Double Emulsion Droplets for Directed Evolution by Zinchenko, Anastasia, Devenish, Sean R. A., Kintses, Balint, Colin, Pierre-Yves, Fischlechner, Martin, Hollfelder, Florian

“…Directed evolution relies on iterative cycles of randomization and selection. The outcome of an artificial evolution experiment is crucially dependent on (i)…”
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Accessing unexplored regions of sequence space in directed enzyme evolution via insertion/deletion mutagenesis by Emond, Stephane, Petek, Maya, Kay, Emily J., Heames, Brennen, Devenish, Sean R. A., Tokuriki, Nobuhiko, Hollfelder, Florian

“…Insertions and deletions (InDels) are frequently observed in natural protein evolution, yet their potential remains untapped in laboratory evolution. Here we…”
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Microfluidic antibody affinity profiling of alloantibody-HLA interactions in human serum by Schneider, Matthias M., Scheidt, Tom, Priddey, Ashley J., Xu, Catherine K., Hu, Mengsha, Meisl, Georg, Devenish, Sean R.A., Dobson, Christopher M., Kosmoliaptsis, Vasilis, Knowles, Tuomas P.J.

“…Antibody profiling is a fundamental component of understanding the humoral response in a wide range of disease areas. Most currently used approaches operate by…”
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Microfluidic droplets: new integrated workflows for biological experiments by Kintses, Balint, van Vliet, Liisa D, Devenish, Sean RA, Hollfelder, Florian

“…Miniaturization of the classical test tube to picoliter dimensions is possible in monodisperse water-in-oil droplets that are generated in microfluidic…”
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A Fully Unsupervised Compartment-on-Demand Platform for Precise Nanoliter Assays of Time-Dependent Steady-State Enzyme Kinetics and Inhibition by Gielen, Fabrice, van Vliet, Liisa, Koprowski, Bartosz T, Devenish, Sean R. A, Fischlechner, Martin, Edel, Joshua B, Niu, Xize, deMello, Andrew J, Hollfelder, Florian

“…The ability to miniaturize biochemical assays in water-in-oil emulsion droplets allows a massive scale-down of reaction volumes, so that high-throughput…”
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Serological fingerprints link antiviral activity of therapeutic antibodies to affinity and concentration by Fiedler, Sebastian, Devenish, Sean R. A., Morgunov, Alexey S., Ilsley, Alison, Ricci, Francesco, Emmenegger, Marc, Kosmoliaptsis, Vasilis, Theel, Elitza S., Mills, John R., Sholukh, Anton M., Aguzzi, Adriano, Iwasaki, Akiko, Lynn, Andrew K., Knowles, Tuomas P. J.

“…The effectiveness of therapeutic monoclonal antibodies (mAbs) against variants of the SARS-CoV-2 virus is highly variable. As target recognition of mAbs relies…”
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Antibody Affinity Governs the Inhibition of SARS-CoV‑2 Spike/ACE2 Binding in Patient Serum by Fiedler, Sebastian, Piziorska, Monika A., Denninger, Viola, Morgunov, Alexey S., Ilsley, Alison, Malik, Anisa Y., Schneider, Matthias M., Devenish, Sean R. A., Meisl, Georg, Kosmoliaptsis, Vasilis, Aguzzi, Adriano, Fiegler, Heike, Knowles, Tuomas P. J.

“…The humoral immune response plays a key role in suppressing the pathogenesis of SARS-CoV-2. The molecular determinants underlying the neutralization of the…”
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Plant DHDPR forms a dimer with unique secondary structure features that preclude higher-order assembly by Watkin, Serena A J, Keown, Jeremy R, Richards, Eric, Goldstone, David C, Devenish, Sean R A, Grant Pearce, F

“…Dihydrodipicolinate reductase (DHDPR) catalyses the second reaction in the diaminopimelate pathway of lysine biosynthesis in bacteria and plants. In contrast…”
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A simple method to evaluate the biochemical compatibility of oil/surfactant mixtures for experiments in microdroplets by Kaltenbach, Miriam, Devenish, Sean R A, Hollfelder, Florian

“…The enormous reduction of assay volume afforded by compartmentalization into picolitre water-in-oil droplets is an exciting prospect for high-throughput…”
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Microfluidic Antibody Affinity Profiling Reveals the Role of Memory Reactivation and Cross-Reactivity in the Defense Against SARS-CoV‑2 by Denninger, Viola, Xu, Catherine K, Meisl, Georg, Morgunov, Alexey S, Fiedler, Sebastian, Ilsley, Alison, Emmenegger, Marc, Malik, Anisa Y, Piziorska, Monika A, Schneider, Matthias M, Devenish, Sean R. A, Kosmoliaptsis, Vasilis, Aguzzi, Adriano, Fiegler, Heike, Knowles, Tuomas P. J

“…Recent efforts in understanding the course and severity of SARS-CoV-2 infections have highlighted both potentially beneficial and detrimental effects of…”
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NMR Studies Uncover Alternate Substrates for Dihydrodipicolinate Synthase and Suggest That Dihydrodipicolinate Reductase Is Also a Dehydratase by Devenish, Sean R. A, Blunt, John W, Gerrard, Juliet A

“…Despite extensive effort, the drug target dihydrodipicolinate synthase (DHDPS) continues to evade effective inhibition. We used NMR spectroscopy to examine the…”
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A single mutation in the core domain of the lac repressor reduces leakiness by Gatti-Lafranconi, Pietro, Dijkman, Willem P, Devenish, Sean R A, Hollfelder, Florian

“…The lac operon provides cells with the ability to switch from glucose to lactose metabolism precisely when necessary. This metabolic switch is mediated by the…”
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Crystal structure and kinetic study of dihydrodipicolinate synthase from Mycobacterium tuberculosis by Kefala, Georgia, Evans, Genevieve L, Griffin, Michael D W, Devenish, Sean R A, Pearce, F Grant, Perugini, Matthew A, Gerrard, Juliet A, Weiss, Manfred S, Dobson, Renwick C J

“…The three-dimensional structure of the enzyme dihydrodipicolinate synthase (KEGG entry Rv2753c, EC 4.2.1.52) from Mycobacterium tuberculosis (Mtb-DHDPS) was…”
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