Production, purification and chemical characterization of the catecholate siderophore from potent probiotic strains of Bacillus spp

The aim of the present study was to characterize the probiotic qualities of Bacillus isolates and study their siderophore prior to possible siderophoregenic probiotic application for iron nutrition in animals and humans. Bacillus strains were selectively isolated from dairy waste and mango pulp wast...

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Bibliographic Details
Published in:	Bioresource technology Vol. 100; no. 1; pp. 368 - 373
Main Authors:	Patel, Anil K., Deshattiwar, Maroti K., Chaudhari, Bhushan L., Chincholkar, Sudhir B.
Format:	Journal Article
Language:	English
Published:	Kidlington Elsevier Ltd 2009 [New York, NY]: Elsevier Ltd Elsevier
Subjects:	2,3-dihydroxybenzoic acid antibacterial properties antibiotic resistance Bacillus Bacillus (bacteria) Bacillus - chemistry Bacillus - classification Bacillus - isolation & purification Bacillus megaterium Bacillus subtilis Biological and medical sciences Catecholate Catechols - chemistry Catechols - isolation & purification competitive exclusion dietary minerals dietary supplements Escherichia coli food processing wastes Fundamental and applied biological sciences. Psychology intestinal microorganisms iron isolation lactic acid bacteria Mangifera indica Micrococcus flavus molecular sequence data nucleotide sequences Probiotic probiotics Probiotics - chemistry Probiotics - isolation & purification ribosomal DNA Siderophore siderophores Siderophores - chemistry Siderophores - isolation & purification Species Specificity Staphylococcus aureus strain differences strains Probiotic 2,3-dihydroxybenzoic acid Siderophore Catecholate Bacillus Purification Bacillales Acids Bacteria Chemical properties Bacillaceae
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Summary:	The aim of the present study was to characterize the probiotic qualities of Bacillus isolates and study their siderophore prior to possible siderophoregenic probiotic application for iron nutrition in animals and humans. Bacillus strains were selectively isolated from dairy waste and mango pulp waste. Best two siderophore positive isolates, JHT3 and DET6 showed high homology with Bacillus megaterium (98%) and B. subtilis (99%), respectively, using partial 16S-rRNA sequencing and biochemical characterization. These isolates produced catecholate type of siderophore under iron stressed conditions and were screened for probiotic properties as per WHO and FAO guidelines. Spores of these strains showed excellent tolerance in partially simulated gastrointestinal tract conditions and exhibited antimicrobial activity against organisms such as Staphylococcus aureus, Micrococcus flavus and Escherichia coli. Importantly, these isolates were susceptible to the most of the antibiotics tested, in conflict that they would not donate resistance determinants if administered in the form of probiotic preparations.
Bibliography:	http://dx.doi.org/10.1016/j.biortech.2008.05.008 ObjectType-Article-2 SourceType-Scholarly Journals-1 ObjectType-Feature-1 content type line 23 ObjectType-Article-1 ObjectType-Feature-2
ISSN:	0960-8524 1873-2976
DOI:	10.1016/j.biortech.2008.05.008