Characterization and experimental infection of Flexibacter maritimus (Wakabayashi et al. 1986) in hatcheries of post-larvae of Litopenaeus vannamei Boone, 1931

Characterization and experimental infection of Flexibacter maritimus (Wakabayashi et al. 1986) in hatcheries of post-larvae of Litopenaeus vannamei Boone, 1931

A preliminary study to characterize filamentous bacteria, whose presence is related to high mortality of Litopenaeus vannamei larvae cultured in Santa Catarina State, Brazil, is reported. The extract of infected larvae was diluted in different concentrations, cultured in marine agar (Difco, Marine A...

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Published in:Brazilian journal of biology Vol. 68; no. 1; pp. 173 - 177
Main Authors: Mouriño, J L P, Vinatea, L, Buglione-Neto, C, Ramirez, C T, Vieira, F N, Pedrotti, F, Martins, M L, Derner, R B, Aguilar, M A, Beltrame, E
Format: Journal Article
Language:English
Published: Brazil Instituto Internacional de Ecologia 01-02-2008
Subjects:
Animals
BIOLOGY
experimental infection
Flexibacter maritimus
Gram-Negative Bacteria - pathogenicity
hatchery
Larva - microbiology
Litopenaeus vannamei
Penaeidae - growth & development
Penaeidae - microbiology
Survival Analysis
Time Factors
hatchery
infecção experimental
experimental infection
Litopenaeus vannamei
pós-larvas
Flexibacter maritimus
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Summary:A preliminary study to characterize filamentous bacteria, whose presence is related to high mortality of Litopenaeus vannamei larvae cultured in Santa Catarina State, Brazil, is reported. The extract of infected larvae was diluted in different concentrations, cultured in marine agar (Difco, Marine Agar 2216) and incubated at 30 degrees C for 48 hours. The biochemical characterization included hydrolytic reactions of starch, gelatin and tyrosine, growth in TCBS agar, growth in 0 and 37 per thousand salinity, pigment production in tyrosine agar, production of H2S, nitrate reduction, congo red reaction, oxidase and catalase. The isolated bacteria belong to the species Flexibacter maritimus, Gram-negative bacilli of 0.4-0.5 microm width and 15 microm length. Experiments were carried out on pathogenicity of F. maritimus in post-larvae of L. vannamei. Survival and symptoms in L. vannamei post-larvae 24 hours after inoculation with F. maritimus and its growth in marine agar were evaluated. Mortality was detected around 92,5% as well as symptoms like melanized lesions in several parts of body, discolouration of gills, bad formation of appendages and of the last abdominal segment, low motility and feeding reduction. The experimental infection results suggested that isolated bacteria of the genus Flexibacter are pathogenic to the shrimp Litopenaeus vannamei post-larvae.
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ISSN:1519-6984
1678-4375
1519-6984
DOI:10.1590/S1519-69842008000100025