Differentiation between Campylobacter hyoilei and Campylobacter coli using genotypic and phenotypic analyses

MS Dep, GL Mendz, MA Trend, PJ Coloe, BN Fry and V Korolik Applied Biology and Biotechnology, RMIT University, Melbourne, Victoria, Australia Genotypic and phenotypic methods were applied to investigate differences between the closely related species Campylobacter hyoilei and Campylobacter coli. A u...

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Bibliographic Details
Published in:	International journal of systematic and evolutionary microbiology Vol. 51; no. 3; pp. 819 - 826
Main Authors:	Dep, MS, Mendz, GL, Trend, MA, Coloe, PJ, Fry, BN, Korolik, V
Format:	Journal Article
Language:	English
Published:	Soc General Microbiol 01-05-2001
Subjects:	Campylobacter coli Campylobacter hyoilei
Online Access:	Get full text
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Summary:	MS Dep, GL Mendz, MA Trend, PJ Coloe, BN Fry and V Korolik Applied Biology and Biotechnology, RMIT University, Melbourne, Victoria, Australia Genotypic and phenotypic methods were applied to investigate differences between the closely related species Campylobacter hyoilei and Campylobacter coli. A unique DNA sequence from C. hyoilei was used to design a specific PCR assay that amplified a DNA product of 383 bp for all C. hyoilei strains, but not other Campylobacter species, including C. coli. The PCR assay could detect 100 fg pure C. hyoilei DNA, 2 x 10(2) c.f.u. ml(-1) using cultured cells and 8.3 x 10(3) c.f.u. 0.1 g(-1) in faeces. The C. hyoilei sequence utilized for specific detection and identification of this species showed similarities to sequences from bacteriophages Mu, P2 and 186, suggesting lysogination of the ancestral C. hyoilei genome. Activities of a set of 15 enzymes that participate in a variety of cellular functions, including biosynthesis, catabolism, energy generation, maintenance of redox balance and phosphate utilization, were tested using sets of strains of C. hyoilei and C. coli. Comparison of mean rates of enzyme activities revealed significant differences between species in the values determined for seven of these activities. Both the genetic and phenotypic data indicate that C. hyoilei is a unique Campylobacter species.
Bibliography:	ObjectType-Article-2 SourceType-Scholarly Journals-1 ObjectType-Feature-1 content type line 23
ISSN:	1466-5026 1466-5034
DOI:	10.1099/00207713-51-3-819