Search Results - "Dennis L. Barton"

Cloning and characterization of an acyl-CoA-dependent diacylglycerol acyltransferase 1 (DGAT1) gene from Tropaeolum majus, and a study of the functional motifs of the DGAT protein using site-directed mutagenesis to modify enzyme activity and oil content by Xu, Jingyu, Francis, Tammy, Mietkiewska, Elzbieta, Giblin, E Michael, Barton, Dennis L, Zhang, Yan, Zhang, Meng, Taylor, David C

“…A full-length cDNA encoding a putative diacylglycerol acyltransferase 1 (DGAT1, EC 2.3.1.20) was obtained from Tropaeolum majus (garden nasturtium). The…”
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Increase in nervonic acid content in transformed yeast and transgenic plants by introduction of a Lunaria annua L. 3-ketoacyl-CoA synthase (KCS) gene by Guo, Yiming, Mietkiewska, Elzbieta, Francis, Tammy, Katavic, Vesna, Brost, Jennifer M, Giblin, Michael, Barton, Dennis L, Taylor, David C

“…Nervonic acid is a Very Long-Chain Monounsaturated Fatty Acid (VLCMFA), 24:1 Δ15 (cis-tetracos-15-enoic acid) found in the seed oils of Lunaria annua, borage,…”
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Seed-Specific Over-Expression of an Arabidopsis cDNA Encoding a Diacylglycerol Acyltransferase Enhances Seed Oil Content and Seed Weight by Colette Jako, Kumar, Arvind, Yangdou Wei, Zou, Jitao, Dennis L. Barton, Giblin, E. Michael, Patrick S. Covello, Taylor, David C.

“…We recently reported the cloning and characterization of an Arabidopsis (ecotype Columbia) diacylglycerol acyltransferase cDNA (Zou et al., 1999) and found…”
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Cloning and functional characterization of the fatty acid elongase 1 (FAE1) gene from high erucic Crambe abyssinica cv. Prophet by Mietkiewska, Elzbieta, Brost, Jennifer M, Giblin, E Michael, Barton, Dennis L, Taylor, David C

“…A genomic fatty acid elongation 1 (FAE1) clone was isolated from Crambe abyssinica. The genomic clone corresponds to a 1521-bp open reading frame, which…”
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Seed-Specific Heterologous Expression of a Nasturtium FAE Gene in Arabidopsis Results in a Dramatic Increase in the Proportion of Erucic Acid by Elzbieta Mietkiewska, Giblin, E. Michael, Song Wang, Dennis L. Barton, Joan Dirpaul, Jennifer M. Brost, Vesna Katavic, Taylor, David C.

“…The fatty acid elongase [often designated FAE or β-(or 3-) ketoacyl-CoA synthase] is a condensing enzyme and is the first component of the elongation complex…”
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The Plastidic Phosphoglucomutase from Arabidopsis. A Reversible Enzyme Reaction with an Important Role in Metabolic Control by Cyril Periappuram, Lee Steinhauer, Dennis L. Barton, Taylor, David C., Brock Chatson, Zou, Jitao

“…An Arabidopsis cDNA (AtPGMp) encoding the plastidic phosphoglucomutase (PGM) predicted a 623-amino acid protein with an N-terminal sequence typical of a…”
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Restoring enzyme activity in nonfunctional low erucic acid Brassica napus fatty acid elongase 1 by a single amino acid substitution by Katavic, Vesna, Mietkiewska, Elzbieta, Barton, Dennis L., Giblin, E. Michael, Reed, Darwin W., Taylor, David C.

“…Genomic fatty acid elongation 1 (FAE1) clones from high erucic acid (HEA) Brassica napus, Brassica rapa and Brassica oleracea, and low erucic acid (LEA) B…”
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Hairpin-RNA mediated silencing of endogenous FAD2 gene combined with heterologous expression of Crambe abyssinica FAE gene causes an increase in the level of erucic acid in transgenic Brassica carinata seeds by Mietkiewska, Elzbieta, Hoffman, Travis L, Brost, Jennifer M, Giblin, E. Michael, Barton, Dennis L, Francis, Tammy, Zhang, Yan, Taylor, David C

“…The 3'-UTR of the FAD2 gene from Brassica carinata was cloned by PCR and used to prepare an intron-spliced hairpin RNA (ihpRNA) construct. Compared to that of…”
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Increased levels of erucic acid in Brassica carinata by co-suppression and antisense repression of the endogenous FAD2 gene by Jadhav, Ashok, Katavic, Vesna, Marillia, Elizabeth-France, Michael Giblin, E., Barton, Dennis L., Kumar, Arvind, Sonntag, Cory, Babic, Vivijan, Keller, Wilfred A., Taylor, David C.

“…Erucic acid and its derivatives represent important industrial feedstock compounds, and there is an increasing demand for the production of high erucate oils…”
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Biosynthesis of Acyl Lipids Containing Very-Long Chain Fatty Acids in Microspore-Derived and Zygotic Embryos of Brassica napus L. cv Reston by Taylor, David C., Dennis L. Barton, Kevin P. Rioux, Samuel L. Mac Kenzie, Darwin W. Reed, Edward W. Underhill, M. Keith Pomeroy, Weber, Nikolaus

“…Biosynthesis of very long chain (> C18) fatty acids (VLCFAs) and the pathway for their incorporation into acyl lipids was studied in microspore-derived (MD)…”
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Alteration of Seed Fatty Acid Composition by an Ethyl Methanesulfonate-Induced Mutation in Arabidopsis thaliana Affecting Diacylglycerol Acyltransferase Activity by Vesna Katavic, Darwin W. Reed, Taylor, David C., Giblin, E. Michael, Dennis L. Barton, Zou, Jitao, Samuel L. Mac Kenzie, Patrick S. Covello, Kunst, Ljerka

“…In characterizing the enzymes involved in the formation of very long-chain fatty acids (VLCFAs) in the Brassicaceae, we have generated a series of mutants of…”
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Field testing of transgenic rapeseed cv. Hero transformed with a yeast sn-2 acyltransferase results in increased oil content, erucic acid content and seed yield by Taylor, David C, Katavic, Vesna, Zou, Jitao, MacKenzie, Samuel L, Keller, Wilfred A, An, Jing, Friesen, Winnie, Barton, Dennis L, Pedersen, Kalie K, Giblin, E Michael, Ge, Yan, Dauk, Melanie, Sonntag, Cory, Luciw, Tammy, Males, Daryl

“…A major goal of our research is to produce, by genetic manipulation, Brassica napus L. cultivars with higher levels of 22:1 in their seed oil than in present…”
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Triacylglycerol bioassembly in microspore-derived embryos of Brassica napus L. cv Reston by Taylor, D.C, Weber, N, Barton, D.L, Underhill, E.W, Hogge, L.R, Weselake, R.J, Pomeroy, M.K

“…Erucic acid (22:1) was chosen as a marker to study triacylglycerol (TAG) biosynthesis in a Brassica napus L. cv Reston microspore-derived (MD) embryo culture…”
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Gaining insight into the role of serine 282 in B. napus FAE1 condensing enzyme by Katavic, Vesna, Barton, Dennis L., Giblin, E.Michael, Reed, Darwin W., Kumar, Arvind, Taylor, David C.

“…To gain some insight whether there is an absolute requirement for the serine 282 to yield a functional fatty acid elongase 1 condensing enzyme we have…”
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Seed-Specific Heterologous Expression of a Nasturtium FAE Gene in Arabidopsis Results in a Dramatic Increase in the Proportion of Erucic Acid1 by Mietkiewska, Elzbieta, Giblin, E Michael, Wang, Song, Barton, Dennis L

“…The fatty acid elongase [often designated FAE or beta-(or 3-) ketoacyl-CoA synthase] is a condensing enzyme and is the first component of the elongation…”
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Induction of Lipid and Oleosin Biosynthesis by (+)-Abscisic Acid and Its Metabolites in Microspore-Derived Embryos of Brassica napus L. cv Reston: Biological Responses in the Presence of 8′-Hydroxyabscisic Acid by Zou, Jitao, Garth D. Abrams, Dennis L. Barton, Taylor, David C., M. Keith Pomeroy, Suzanne R. Abrams

“…Microspore-derived (MD) embryos of Brassica napus L. cv Reston were used to test the effects of (+)-abscisic acid ([(+)-ABA]) and its metabolites,…”
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Improving erucic acid content in rapeseed through biotechnology : What can the Arabidopsis FAE1 and the yeast SLC1-1 genes contribute ? by KATAVIC, Vesna, FRIESEN, Winnie, MALES, Daryl, TAYLOR, David C, BARTON, Dennis L, GOSSEN, Kalie K, GIBLIN, E. Michael, LUCIW, Tammy, JING AN, JITAO ZOU, MACKENZIE, Samuel L, KELLER, Wilfred A

“…The main goal of our research is to produce, by genetic manipulation, Brassica napus L. cultivars with higher amounts of 22:1 in their seed oil than in present…”
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Microsomal lyso-phosphatidic acid acyltransferase from a Brassica oleracea cultivar incorporates erucic acid into the sn-2 position of seed triacylglycerols by Taylor, D.C. (National Research Council of Canada, Saskatoon, Saskatchewan, Canada.), Barton, D.L, Giblin, E.M, MacKenzie, S.L, Berg, C.G.J. van den, McVetty, P.B.E

“…Developing seeds from Brassica oleracea (L.) var botrytis cv Sesam were examined for the ability to biosynthesize and incorporate erucic acid into…”
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A Teesdalia nudicaulis FAE1 complements the fae1 mutation in transgenic Arabidopsis thaliana plants and shows a preference for elongating oleic acid to eicosenoic acid by Mietkiewska, Elzbieta, Brost, Jennifer M., Giblin, E. Michael, Barton, Dennis L., Taylor, David C.

“…Using a PCR-based approach, a genomic clone of a putative fatty acid elongase ( FAE) showing high similarity to plant microsomal fatty acid elongases, was…”
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Modification of seed oil content and acyl composition in the Brassicaceae by expression of a yeast sn-2 acyltransferase gene by Zou, J. (National Research Council of Canada, Saskatoon, Saskatchewan, Canada.), Katavic, V, Giblin, E.M, Barton, D.L, MacKenzie, S.L, Keller, W.A, Hu, X, Taylor, D.C

“…A putative yeast sn-2 acyltransferase gene (SLC1-1), reportedly a variant acyltransferase that suppresses a genetic defect in sphingolipid long-chain base…”
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