Detection of chronic wasting disease in mule and white-tailed deer by RT-QuIC analysis of outer ear

Detection of chronic wasting disease in mule and white-tailed deer by RT-QuIC analysis of outer ear

Efforts to contain the spread of chronic wasting disease (CWD), a fatal, contagious prion disease of cervids, would be aided by the availability of additional diagnostic tools. RT-QuIC assays allow ultrasensitive detection of prion seeds in a wide variety of cervid tissues, fluids and excreta. The b...

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Bibliographic Details
Published in:Scientific reports Vol. 11; no. 1; p. 7702
Main Authors: Ferreira, Natalia C., Charco, Jorge M., Plagenz, Jakob, Orru, Christina D., Denkers, Nathanial D., Metrick, Michael A., Hughson, Andrew G., Griffin, Karen A., Race, Brent, Hoover, Edward A., Castilla, Joaquín, Nichols, Tracy A., Miller, Michael W., Caughey, Byron
Format: Journal Article
Language:English
Published: London Nature Publishing Group UK 08-04-2021
Nature Publishing Group
Nature Portfolio
Subjects:
631/1647
631/326
631/378/1689
692/53
692/699
692/699/255
692/699/375
Biopsy
Cervidae
Chronic wasting disease
Deer
Ear
Humanities and Social Sciences
Iron oxides
Lymph nodes
Lymphoid tissue
multidisciplinary
Odocoileus virginianus
Rectum
Science
Science (multidisciplinary)
Transmissible spongiform encephalopathy
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Summary:Efforts to contain the spread of chronic wasting disease (CWD), a fatal, contagious prion disease of cervids, would be aided by the availability of additional diagnostic tools. RT-QuIC assays allow ultrasensitive detection of prion seeds in a wide variety of cervid tissues, fluids and excreta. The best documented antemortem diagnostic test involving RT-QuIC analysis targets lymphoid tissue in rectal biopsies. Here we have tested a more easily accessed specimen, ear pinna punches, using an improved RT-QuIC assay involving iron oxide magnetic extraction to detect CWD infections in asymptomatic mule and white-tailed deer. Comparison of multiple parts of the ear pinna indicated that a central punch spanning the auricular nerve provided the most consistent detection of CWD infection. When compared to results obtained from gold-standard retropharyngeal lymph node specimens, our RT-QuIC analyses of ear samples provided apparent diagnostic sensitivity (81%) and specificity (91%) that rivaled, or improved upon, those observed in previous analyses of rectal biopsies using RT-QuIC. These results provide evidence that RT-QuIC analysis of ear pinna punches may be a useful approach to detecting CWD infections in cervids.
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ISSN:2045-2322
2045-2322
DOI:10.1038/s41598-021-87295-8