Molecular cloning of the OMP19 gene from Brucella melitensis strain H38 and its antigenicity compared to that of commercial OMP19

Molecular cloning of the OMP19 gene from Brucella melitensis strain H38 and its antigenicity compared to that of commercial OMP19

Brucellosis is a worldwide zoonosis, that can still be classified as endemic despite its ancient origins which causes economic losses and public health problems. Although effectively controlled by vaccination in animals, there is currently no vaccine for use in humans. Outer Membrane Proteins (OMP)...

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Bibliographic Details
Published in:Polish journal of veterinary sciences Vol. 25; no. 4; pp. 561 - 569
Main Authors: Uslu, A, Sanioglu Golen, G, Agah Tekindal, M, Sakmanoglu, A, Sayın, Z, Denizli, O, Gok, A, Erganis, O
Format: Journal Article
Language:English
Published: Germany Polish Academy of Sciences, Committee of Veterinary Sciences 01-12-2022
Subjects:
Animals
Antibodies
Antigenicity
Antigens
Antigens, Bacterial
Bacteria
Bacterial infections
Bacterial Outer Membrane Proteins - genetics
Bacterial Outer Membrane Proteins - metabolism
Brucella
Brucella melitensis - genetics
Brucella melitensis - metabolism
Brucellosis
Brucellosis - prevention & control
Brucellosis - veterinary
Cloning
Cloning, Molecular
E coli
Female
Humans
Immunogenicity
Membrane proteins
Mice
Mice, Inbred BALB C
Outer membrane proteins
Pets
Pregnancy
Protein expression
Proteins
Public health
Sheep
Vaccination
Vaccines
Zoonoses
United States > US
rOMP19 antigen
recombinant protein
Brucella melitensis
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Summary:Brucellosis is a worldwide zoonosis, that can still be classified as endemic despite its ancient origins which causes economic losses and public health problems. Although effectively controlled by vaccination in animals, there is currently no vaccine for use in humans. Outer Membrane Proteins (OMP) that play an active immunogenic and protective role in the Brucellae family. OMP19 is present in all Brucella species as a surface antigen and is a potent immunogen responsible for Brucellosis intracellular infection. For this reason, the study was aimed to be used safely as a potential recombinant vaccine candidate against all Brucella infections, especially in humans and pregnant animals. This study evaluated a Brucella lipoprotein antigen, i.e. 19 kilodalton (kDa) outer membrane protein (OMP19), which was amplified and cloned into the pETSUMO vector system. The immunogenic power of the purified recombinant OMP19 antigen against brucellosis was compared with that of OMP19 (Raybiotech Inc, USA) in a mouse model and the obtained rOMP19 antigen was found to be similar to the commercially available recombinant protein.
ISSN:1505-1773
2300-2557
DOI:10.24425/pjvs.2022.143539