A novel high-throughput method for kinetic characterisation of anaerobic bioproduction strains, applied to Clostridium kluyveri

A novel high-throughput method for kinetic characterisation of anaerobic bioproduction strains, applied to Clostridium kluyveri

Hexanoic acid (HA), also called caproic acid, can be used as an antimicrobial agent and as a precursor to various chemicals, such as fuels, solvents and fragrances. HA can be produced from ethanol and acetate by the mesophilic anaerobic bacterium Clostridium kluyveri , via two successive elongation...

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Bibliographic Details
Published in:Scientific reports Vol. 8; no. 1; pp. 9724 - 13
Main Authors: Candry, Pieter, Van Daele, Timothy, Denis, Kyrina, Amerlinck, Youri, Andersen, Stephen J., Ganigué, Ramon, Arends, Jan B. A., Nopens, Ingmar, Rabaey, Korneel
Format: Journal Article
Language:English
Published: London Nature Publishing Group UK 27-06-2018
Nature Publishing Group
Subjects:
631/1647/2234
631/326/2522
631/61/168
Acetic acid
Acids
Antimicrobial agents
Butyric acid
Clostridium
Data collection
Data processing
Ethanol
Growth kinetics
Growth rate
Humanities and Social Sciences
multidisciplinary
Saturation index
Science
Science (multidisciplinary)
Toxicity
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Summary:Hexanoic acid (HA), also called caproic acid, can be used as an antimicrobial agent and as a precursor to various chemicals, such as fuels, solvents and fragrances. HA can be produced from ethanol and acetate by the mesophilic anaerobic bacterium Clostridium kluyveri , via two successive elongation steps over butyrate. A high-throughput anaerobic growth curve technique was coupled to a data analysis framework to assess growth kinetics for a range of substrate and product concentrations. Using this method, growth rates and several kinetic parameters were determined for C . kluyveri . A maximum growth rate (µ max ) of 0.24 ± 0.01 h −1 was found, with a half-saturation index for acetic acid (K S,AA ) of 3.8 ± 0.9 mM. Inhibition by butyric acid occurred at of 124.7 ± 5.7 mM (K I,BA ), while the final product, HA, linearly inhibited growth with complete inhibition above 91.3 ± 10.8 mM (K HA of 10.9*10 −3  ± 1.3*10 −3  mM −1 ) at pH = 7, indicating that the hexanoate anion also exerts toxicity. These parameters were used to create a dynamic mass-balance model for bioproduction of HA. By coupling data collection and analysis to this modelling framework, we have produced a powerful tool to assess the kinetics of anaerobic micro-organisms, demonstrated here with C . kluyveri , in order further explore the potential of micro-organisms for chemicals production.
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ISSN:2045-2322
2045-2322
DOI:10.1038/s41598-018-27594-9