Enhancing scanning electrochemical microscopy's potential to probe dynamic co-culture systems via hyperspectral assisted-imaging

Precise determination of boundaries in co-culture systems is difficult to achieve with scanning electrochemical microscopy alone. Thus, biological scanning electrochemical microscope platforms generally consist of a scanning electrochemical microscope positioner mounted on the stage of an inverted m...

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Bibliographic Details
Published in:	Analyst (London) Vol. 147; no. 11; p. 2396
Main Authors:	Goines, Sondrica, Deng, Mingchu, Glasscott, Matthew W, Leung, Justin W C, Dick, Jeffrey E
Format:	Journal Article
Language:	English
Published:	England 30-05-2022
Subjects:	Coculture Techniques Lasers Microscopy, Electrochemical, Scanning Microscopy, Fluorescence - methods Optical Imaging
Online Access:	Get more information
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Summary:	Precise determination of boundaries in co-culture systems is difficult to achieve with scanning electrochemical microscopy alone. Thus, biological scanning electrochemical microscope platforms generally consist of a scanning electrochemical microscope positioner mounted on the stage of an inverted microscope for correlated electrochemical and optical imaging. Use of a fluorescence microscope allows for site-specific fluorescence labeling to obtain more clearly resolved spatial and electrochemical data. Here, we construct a unique hyperspectral assisted-biological scanning electrochemical microscope platform to widen the scope of biological imaging. Specifically, we incorporate a variable fluorescence bandpass source into a biological scanning electrochemical microscope platform for simultaneous optical, spectral, and electrochemical imaging. Not only does this platform serve as a cost-effective alternative to white light laser imaging, but additionally it provides multi-functional analysis of biological samples. Here, we demonstrate the efficacy of our platform to discern the electrochemical contribution of site-specific cells by optically and spectroscopically resolving boundaries as well as cell types within a complex biological system.
ISSN:	1364-5528
DOI:	10.1039/d2an00319h