Search Results - "Demura, Makoto"

Real-time identification of two substrate-binding intermediates for the light-driven sodium pump rhodopsin by Kato, Tomoya, Tsukamoto, Takashi, Demura, Makoto, Kikukawa, Takashi

“…Membrane transport proteins undergo critical conformational changes during substrate uptake and release, as the substrate-binding site is believed to switch…”
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Direct Detection of the Substrate Uptake and Release Reactions of the Light-Driven Sodium-Pump Rhodopsin by Murabe, Keisuke, Tsukamoto, Takashi, Aizawa, Tomoyasu, Demura, Makoto, Kikukawa, Takashi

“…For membrane transporters, substrate uptake and release reactions are major events during their transport cycles. Despite the functional importance of these…”
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Replaceability of Schiff base proton donors in light-driven proton pump rhodopsins by Sasaki, Syogo, Tamogami, Jun, Nishiya, Koki, Demura, Makoto, Kikukawa, Takashi

“…Many H+-pump rhodopsins conserve “H+ donor” residues in cytoplasmic (CP) half channels to quickly transport H+ from the CP medium to Schiff bases at the center…”
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Preference of Proteomonas sulcata anion channelrhodopsin for NO3− revealed using a pH electrode method by Kikuchi, Chihiro, Kurane, Hina, Watanabe, Takuma, Demura, Makoto, Kikukawa, Takashi, Tsukamoto, Takashi

“…Ion channel proteins are physiologically important molecules in living organisms. Their molecular functions have been investigated using electrophysiological…”
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Mutations conferring SO42− pumping ability on the cyanobacterial anion pump rhodopsin and the resultant unique features of the mutant by Doi, Yuhei, Watanabe, Jo, Nii, Ryota, Tsukamoto, Takashi, Demura, Makoto, Sudo, Yuki, Kikukawa, Takashi

“…Membrane transport proteins can be divided into two types: those that bind substrates in a resting state and those that do not. In this study, we demonstrate…”
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Implications for the Light-Driven Chloride Ion Transport Mechanism of Nonlabens marinus Rhodopsin 3 by Its Photochemical Characteristics by Tsukamoto, Takashi, Yoshizawa, Susumu, Kikukawa, Takashi, Demura, Makoto, Sudo, Yuki

“…Several new retinal-based photoreceptor proteins that act as light-driven electrogenic halide ion pumps have recently been discovered. Some of them, called…”
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Transient Resonance Raman Spectroscopy of a Light-Driven Sodium-Ion-Pump Rhodopsin from Indibacter alkaliphilus by Kajimoto, Kousuke, Kikukawa, Takashi, Nakashima, Hiroki, Yamaryo, Haruki, Saito, Yuta, Fujisawa, Tomotsumi, Demura, Makoto, Unno, Masashi

“…Sodium-ion-pump rhodopsin (NaR) is a microbial rhodopsin that transports Na+ during its photocycle. Here we explore the photocycle mechanism of NaR from…”
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Potent bactericidal activity of reduced cryptdin-4 derived from its hydrophobicity and mediated by bacterial membrane disruption by Sato, Yuji, Wang, Yi, Song, Yuchi, Geng, Weiming, Yan, Shaonan, Nakamura, Kiminori, Kikukawa, Takashi, Demura, Makoto, Ayabe, Tokiyoshi, Aizawa, Tomoyasu

“…Defensin is a cysteine-rich antimicrobial peptide with three disulphide bonds under normal oxidative conditions. Cryptdin-4 (Crp4) is a defensin secreted by…”
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Photochemical study of a cyanobacterial chloride-ion pumping rhodopsin by Hasemi, Takatoshi, Kikukawa, Takashi, Watanabe, Yumi, Aizawa, Tomoyasu, Miyauchi, Seiji, Kamo, Naoki, Demura, Makoto

“…Mastigocladopsis repens halorhodopsin (MrHR) is a Cl−-pumping rhodopsin that belongs to a distinct cluster far from other Cl− pumps. We investigated its…”
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Protein-Bound Water as the Determinant of Asymmetric Functional Conversion between Light-Driven Proton and Chloride Pumps by Muroda, Kosuke, Nakashima, Keisuke, Shibata, Mikihiro, Demura, Makoto, Kandori, Hideki

“…Bacteriorhodopsin (BR) and halorhodopsin (HR) are light-driven outward proton and inward chloride pumps, respectively. They have similar protein architecture,…”
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Functional importance of the oligomer formation of the cyanobacterial H+ pump Gloeobacter rhodopsin by Iizuka, Azusa, Kajimoto, Kousuke, Fujisawa, Tomotsumi, Tsukamoto, Takashi, Aizawa, Tomoyasu, Kamo, Naoki, Jung, Kwang-Hwan, Unno, Masashi, Demura, Makoto, Kikukawa, Takashi

“…Many microbial rhodopsins self-oligomerize, but the functional consequences of oligomerization have not been well clarified. We examined the effects of…”
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Expression, purification and characterization of the recombinant cysteine-rich antimicrobial peptide snakin-1 in Pichia pastoris by Kuddus, Md. Ruhul, Rumi, Farhana, Tsutsumi, Motosuke, Takahashi, Rika, Yamano, Megumi, Kamiya, Masakatsu, Kikukawa, Takashi, Demura, Makoto, Aizawa, Tomoyasu

“…Snakin-1 (SN-1) is a small cysteine-rich plant antimicrobial peptide with broad spectrum antimicrobial activity which was isolated from potato (Solanum…”
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Implications for the impairment of the rapid channel closing of Proteomonas sulcata anion channelrhodopsin 1 at high Cl− concentrations by Tsukamoto, Takashi, Kikuchi, Chihiro, Suzuki, Hiromu, Aizawa, Tomoyasu, Kikukawa, Takashi, Demura, Makoto

“…Natural anion channelrhodopsins (ACRs) have recently received increased attention because of their effectiveness in optogenetic manipulation for neuronal…”
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Role of S-palmitoylation on IFITM5 for the interaction with FKBP11 in osteoblast cells by Tsukamoto, Takashi, Li, Xianglan, Morita, Hiromi, Minowa, Takashi, Aizawa, Tomoyasu, Hanagata, Nobutaka, Demura, Makoto

“…Recently, one of the interferon-induced transmembrane (IFITM) family proteins, IFITM3, has become an important target for the activity against influenza A…”
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Photochemical characterization of actinorhodopsin and its functional existence in the natural host by Nakamura, Shintaro, Kikukawa, Takashi, Tamogami, Jun, Kamiya, Masakatsu, Aizawa, Tomoyasu, Hahn, Martin W., Ihara, Kunio, Kamo, Naoki, Demura, Makoto

“…Actinorhodopsin (ActR) is a light-driven outward H+ pump. Although the genes of ActRs are widely spread among freshwater bacterioplankton, there are no prior…”
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Salt bridge in the conserved His–Asp cluster in Gloeobacter rhodopsin contributes to trimer formation by Tsukamoto, Takashi, Kikukawa, Takashi, Kurata, Takuro, Jung, Kwang-Hwan, Kamo, Naoki, Demura, Makoto

“…► GR solubilized with DDM shows the pH-dependent trimer/monomer transition. ► His87–Asp121 salt bridge contributes to the trimer formation. ► His87 itself is…”
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Enhanced expression of cysteine‐rich antimicrobial peptide snakin‐1 in Escherichia coli using an aggregation‐prone protein coexpression system by Kuddus, Md. Ruhul, Yamano, Megumi, Rumi, Farhana, Kikukawa, Takashi, Demura, Makoto, Aizawa, Tomoyasu

“…Snakin‐1 (SN‐1) is a cysteine‐rich plant antimicrobial peptide and the first purified member of the snakin family. SN‐1 shows potent activity against a wide…”
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Photocycle of Sensory Rhodopsin II from Halobacterium salinarum (HsSRII): Mutation of D103 Accelerates M Decay and Changes the Decay Pathway of a 13‐cis O‐like Species by Dai, Gang, Geng, Xiong, Chaoluomeng, Tamogami, Jun, Kikukawa, Takashi, Demura, Makoto, Kamo, Naoki, Iwasa, Tatsuo

“…Aspartic acid 103 (D103) of sensory rhodopsin II from Halobacterium salinarum (HsSRII, or also called phoborhodopsin) corresponds to D115 of bacteriorhodopsin…”
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Biophysical research in Hokkaido University, Japan by Aizawa, Tomoyasu, Demura, Makoto, Gohara, Kazutoshi, Haga, Hisashi, Ishimori, Koichiro, Kinjo, Masataka, Komatsuzaki, Tamiki, Maenaka, Katsumi, Yao, Min

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Presence of a Haloarchaeal Halorhodopsin-Like Cl− Pump in Marine Bacteria by Nakajima, Yu, Tsukamoto, Takashi, Kumagai, Yohei, Ogura, Yoshitoshi, Hayashi, Tetsuya, Song, Jaeho, Kikukawa, Takashi, Demura, Makoto, Kogure, Kazuhiro, Sudo, Yuki, Yoshizawa, Susumu

“…Light-driven ion-pumping rhodopsins are widely distributed among bacteria, archaea, and eukaryotes in the euphotic zone of the aquatic environment. H+-pumping…”
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