Search Results - "Dellinger, Douglas"
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1
Improving CRISPR-Cas specificity with chemical modifications in single-guide RNAs
Published in Nucleic acids research (25-01-2018)“…Abstract CRISPR systems have emerged as transformative tools for altering genomes in living cells with unprecedented ease, inspiring keen interest in…”
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2
Phosphonoacetate Modifications Enhance the Stability and Editing Yields of Guide RNAs for Cas9 Editors
Published in Biochemistry (Easton) (19-12-2023)“…CRISPR gene editing and control systems continue to emerge and inspire novel research and clinical applications. Advances in CRISPR performance such as…”
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3
Streamlined Process for the Chemical Synthesis of RNA Using 2′-O-Thionocarbamate-Protected Nucleoside Phosphoramidites in the Solid Phase
Published in Journal of the American Chemical Society (03-08-2011)“…An improved method for the chemical synthesis of RNA was developed utilizing a streamlined method for the preparation of phosphoramidite monomers and a…”
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4
Solid-Phase Chemical Synthesis of Phosphonoacetate and Thiophosphonoacetate Oligodeoxynucleotides
Published in Journal of the American Chemical Society (29-01-2003)“…Phosphonoacetate and thiophosphonoacetate oligodeoxynucleotides were prepared via a solid-phase synthesis strategy. Under Reformatsky reaction conditions,…”
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5
An electrospray mass spectrometric method for accurate mass determination of highly acid-sensitive phosphoramidites
Published in Rapid communications in mass spectrometry (01-01-2008)“…An accurate mass determination method utilizing electrospray ionization mass spectrometry is described for analysis of several different types of…”
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6
Synthesis and Biochemical Evaluation of Phosphonoformate Oligodeoxyribonucleotides
Published in Journal of the American Chemical Society (19-04-2006)“…Phosphonoformate oligodeoxyribonucleotides were prepared via a solid phase synthesis strategy. The first step in the preparation of appropriate synthons was…”
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7
Solid-Phase Oligodeoxynucleotide Synthesis: A Two-Step Cycle Using Peroxy Anion Deprotection
Published in Journal of the American Chemical Society (05-11-2003)“…A novel solid-phase phosphoramidite based oligodeoxynucleotide two-step synthesis method has been developed. Keys to this method are replacement of the…”
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8
Biochemical properties of phosphonoacetate and thiophosphonoacetate oligodeoxyribonucleotides
Published in Nucleic acids research (15-07-2003)“…Phosphorus‐modified phosphonoacetate and thiophosphonoacetate oligodeoxyribonucleotides were chemically synthesized and their biochemical properties evaluated…”
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9
Synthesis and Biological Activity of Phosphonocarboxylate DNA
Published in Nucleosides, nucleotides & nucleic acids (01-01-2007)“…Oligodeoxynucleotides containing internucleotide phosphonoacetate esters are taken up irreversibly by cells in culture in the absence of cationic lipids. These…”
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10
Synthesis of DNA using a new two-step cycle
Published in Methods in molecular biology (Clifton, N.J.) (2005)“…For the first time a new, two-step method is described for synthesizing deoxyribonucleic acid. The approach uses 5'-carbonate protected…”
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11
Chemically modified guide RNAs enhance CRISPR-Cas genome editing in human primary cells
Published in Nature biotechnology (01-09-2015)“…Improved efficiency of CRISPR/Cas editing with chemically modified synthetic sgRNAs. CRISPR-Cas-mediated genome editing relies on guide RNAs that direct…”
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12
Oligodeoxyribonucleotide analogs functionalized with phosphonoacetate and thiophosphonoacetate diesters
Published in Current protocols in nucleic acid chemistry (01-10-2004)“…Oligodeoxyribonucleotides with phosphonoacetate or thiophosphonoacetate internucleotide linkages can be made in high yield by solid-phase synthesis and possess…”
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13
Studies on the chemical synthesis of oligoribonucleotides and oligodeoxyribonucleotides
Published 01-01-1991“…Oligoribonucleotides (RNA) as targets for chemical synthesis, present a complex problem for standard protecting group methods. The addition of an extra…”
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