Unipolar (Dendritic) Brush Cells Are Morphologically Complex and Require Tbr2 for Differentiation and Migration

Unipolar (Dendritic) Brush Cells Are Morphologically Complex and Require Tbr2 for Differentiation and Migration

Previous studies demonstrated specific expression of transcription factor Tbr2 in unipolar brush cells (UBCs) of the cerebellum during development and adulthood. To further study UBCs and the role of Tbr2 in their development we examined UBC morphology in transgenic mouse lines (reporter and lineage...

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Published in:Frontiers in neuroscience Vol. 14; p. 598548
Main Authors: McDonough, Ashley, Elsen, Gina E, Daza, Ray M, Bachleda, Amelia R, Pizzo, Donald, DelleTorri, Olivia M, Hevner, Robert F
Format: Journal Article
Language:English
Published: Switzerland Frontiers Research Foundation 08-01-2021
Frontiers Media S.A
Subjects:
Antibodies
Antigens
Automation
cell migration
Cerebellum
Dendrites
development
Microscopy
Morphology
Neuroscience
Tbr2
Transgenic mice
Unipolar brush cells
cell migration
Tbr2
development
unipolar brush cells
cerebellum
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Summary:Previous studies demonstrated specific expression of transcription factor Tbr2 in unipolar brush cells (UBCs) of the cerebellum during development and adulthood. To further study UBCs and the role of Tbr2 in their development we examined UBC morphology in transgenic mouse lines (reporter and lineage tracer) and also examined the effects of Tbr2 deficiency in (MGI: ) conditional knock-out (cKO) mice. In reporter and lineage tracer cerebellum, UBCs exhibited more complex morphologies than previously reported including multiple dendrites, bifurcating dendrites, and up to four dendritic brushes. We propose that "dendritic brush cells" (DBCs) may be a more apt nomenclature. In cKO cerebellum, mature UBCs were completely absent. Migration of UBC precursors from rhombic lip to cerebellar cortex and other nuclei was impaired in cKO mice. Our results indicate that UBC migration and differentiation are sensitive to Tbr2 deficiency. To investigate whether UBCs develop similarly in humans as in rodents, we studied Tbr2 expression in mid-gestational human cerebellum. Remarkably, Tbr2 UBC precursors migrate along the same pathways in humans as in rodent cerebellum and disperse to create the same "fountain-like" appearance characteristic of UBCs exiting the rhombic lip.
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Reviewed by: Annalisa Buffo, University of Turin, Italy; Felipe Ortega, Complutense University of Madrid, Spain
Edited by: Benedikt Berninger, King’s College London, United Kingdom
This article was submitted to Neurogenesis, a section of the journal Frontiers in Neuroscience
Present address: Ashley McDonough, Department of Neurology, University of Washington, Seattle, WA, United States; Gina E. Elsen, Northwestern Medicine, Chicago, IL, United States; Ray M. Daza and Robert F. Hevner, Sanford Consortium for Regenerative Medicine, San Diego, CA, United States; Amelia R. Bachleda, Institute for Learning & Brain Sciences, University of Washington, Seattle, WA, United States; Olivia M. DelleTorri, Department of Pathology, University of California, San Diego, CA, United States
ISSN:1662-4548
1662-453X
1662-453X
DOI:10.3389/fnins.2020.598548