Non-invasive 3D time-of-flight imaging technique for tumour volume assessment in subcutaneous models

Subcutaneous tumour xenograft volumes are generally measured using callipers. This method is susceptible to inter- and intra-observer variability and systematic inaccuracies. Non-invasive 3D measurement using ultrasound and magnetic resonance imaging (MRI) have been considered, but require immobiliz...

Full description

Saved in:

Bibliographic Details
Published in:	Laboratory animals (London) Vol. 49; no. 2; pp. 168 - 171
Main Authors:	Delgado San Martin, JA, Worthington, P, Yates, JWT
Format:	Journal Article
Language:	English
Published:	London, England SAGE Publications 01-04-2015
Subjects:	Algorithms Animals Disease Models, Animal Image Processing, Computer-Assisted - instrumentation Image Processing, Computer-Assisted - methods Imaging, Three-Dimensional - instrumentation Imaging, Three-Dimensional - methods Mice Photography Tumor Burden infrared non-invasive xenograft reduction
Online Access:	Get full text
Tags:	Add Tag No Tags, Be the first to tag this record!

Description
Summary:	Subcutaneous tumour xenograft volumes are generally measured using callipers. This method is susceptible to inter- and intra-observer variability and systematic inaccuracies. Non-invasive 3D measurement using ultrasound and magnetic resonance imaging (MRI) have been considered, but require immobilization of the animal. An infrared-based 3D time-of-flight (3DToF) camera was used to acquire a depth map of tumour-bearing mice. A semi-automatic algorithm based on parametric surfaces was applied to estimate tumour volume. Four clay mouse models and 18 tumour-bearing mice were assessed using callipers (applying both prolate spheroid and ellipsoid models) and 3DToF methods, and validated using tumour weight. Inter-experimentalist variability could be up to 25% in the calliper method. Experimental results demonstrated good consistency and relatively low error rates for the 3DToF method, in contrast to biased overestimation using callipers. Accuracy is currently limited by camera performance; however, we anticipate the next generation 3DToF cameras will be able to support the development of a practical system. Here, we describe an initial proof of concept for a non-invasive, non-immobilized, morphology-independent, economical and potentially more precise tumour volume assessment technique. This affordable technique should maximize the datapoints per animal, by reducing the numbers required in experiments and reduce their distress.
Bibliography:	ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 23
ISSN:	0023-6772 1758-1117
DOI:	10.1177/0023677214562653