Bone tissue engineering on amorphous carbonated apatite and crystalline octacalcium phosphate-coated titanium discs

Poor fixation of bone replacement implants, e.g. the artificial hip, in implantation sites with inferior bone quality and quantity may be overcome by the use of implants coated with a cultured living bone equivalent. In this study, we tested, respectively, amorphous carbonated apatite (CA)- and crys...

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Bibliographic Details
Published in:	Biomaterials Vol. 26; no. 25; pp. 5231 - 5239
Main Authors:	Dekker, Robert J., de Bruijn, Joost D., Stigter, Martin, Barrere, Florence, Layrolle, Pierre, van Blitterswijk, Clemens A.
Format:	Journal Article
Language:	English
Published:	Netherlands Elsevier Ltd 01-09-2005
Subjects:	Animals Apatites - chemistry Bone and Bones - anatomy & histology Bone Marrow Cells - cytology Bone Marrow Cells - drug effects Bone tissue engineering Calcification, Physiologic - drug effects Calcium phosphate coating Calcium Phosphates - chemistry Cell Culture Techniques Cell Proliferation - drug effects Coated Materials, Biocompatible - chemistry Coated Materials, Biocompatible - pharmacology Implants, Experimental Male Mesenchymal stem cells Mice Mice, Inbred BALB C Mice, Nude Microscopy, Electron, Scanning Osteogenesis - drug effects Rats Rats, Wistar Spectroscopy, Fourier Transform Infrared Tissue Engineering - methods Titanium - chemistry X-Ray Diffraction Calcium phosphate coating Bone tissue engineering Mesenchymal stem cells
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Summary:	Poor fixation of bone replacement implants, e.g. the artificial hip, in implantation sites with inferior bone quality and quantity may be overcome by the use of implants coated with a cultured living bone equivalent. In this study, we tested, respectively, amorphous carbonated apatite (CA)- and crystalline octacalcium phosphate (OCP)-coated discs for their use in bone tissue engineering. Subcultured rat bone marrow cells were seeded on the substrates and after 7 days of culture, the implants were subcutaneously implanted in nude mice for 4 weeks. After 7 days of culture, the cells had formed a continuous multi-layer that covered the entire surface of the substrates. The amount of cells was visually higher on the crystalline OCP-coated discs compared to the amorphous CA-coated discs. Furthermore, the amorphous CA-coated discs exhibited a visually higher amount of mineralized extracellular matrix compared to the crystalline OCP-coated discs. After 4 weeks of implantation, clear de novo bone formation was observed on all discs with cultured cells. The newly formed bone on the crystalline OCP-coated discs was more organized and revealed a significantly higher volume compared to the amorphous CA-coated discs. The percentage of bone contact with the discs was also significantly higher on the OCP-coated discs. Overall, the results suggest that a crystalline OCP coating is more suitable for bone tissue engineering than an amorphous CA coating.
Bibliography:	ObjectType-Article-2 SourceType-Scholarly Journals-1 ObjectType-Feature-1 content type line 23
ISSN:	0142-9612 1878-5905
DOI:	10.1016/j.biomaterials.2005.01.057