Magnetic activated cell sorting: an effective method for reduction of sperm DNA fragmentation in varicocele men prior to assisted reproductive techniques

Magnetic activated cell sorting: an effective method for reduction of sperm DNA fragmentation in varicocele men prior to assisted reproductive techniques

Summary Semen parameters of varicocele men have been usually suspected to exhibit higher levels of abnormalities including DNA fragmentation, reactive oxygen species (ROS) and apoptotic markers. Negative correlation between increased level of DNA fragmentation and assisted reproductive techniques (A...

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Bibliographic Details
Published in:Andrologia Vol. 47; no. 8; pp. 892 - 896
Main Authors: Degheidy, T., Abdelfattah, H., Seif, A., Albuz, F. K., Gazi, S., Abbas, S.
Format: Journal Article
Language:English
Published: Germany Blackwell Publishing Ltd 01-10-2015
Wiley Subscription Services, Inc
Subjects:
Adult
Apoptosis
Cell Separation - methods
Centrifugation, Density Gradient
DNA Fragmentation
DNA fragmentations
Humans
In Situ Nick-End Labeling
Infertility, Male - etiology
MACs
Magnetics - methods
Male
Middle Aged
Reproductive Techniques, Assisted
Sperm Count
Sperm Motility
Spermatozoa - abnormalities
Spermatozoa - cytology
Spermatozoa - physiology
varicocele
Varicocele - complications
Young Adult
DNA fragmentations
MACs
Apoptosis
varicocele
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Summary:Summary Semen parameters of varicocele men have been usually suspected to exhibit higher levels of abnormalities including DNA fragmentation, reactive oxygen species (ROS) and apoptotic markers. Negative correlation between increased level of DNA fragmentation and assisted reproductive techniques (ART) outcome has been studied by several authors. In the current study, we aim to evaluate the possible value of magnetic activated cell sorting (MACs) technology in reduction of DNA fragmentation in infertile varicocele patients prior to ART. Semen samples, collected from 36 varicocele patients, were prepared by density gradient centrifugation (DGC). Every sample was subsequently divided into two aliquots. One aliquot was kept untouched as pre‐MACs control while the other aliquot was subjected to MACs technique, for depletion of apoptotic spermatozoa, and serves as post‐MACs test. Sperm count, motility and DNA fragmentations were evaluated for both control and test samples. Post‐MACs samples showed no deleterious reduction in total sperm motility (80.64 ± 6.97%) compared with control samples (80.97 ± 7.74%) while sperm DNA fragmentations were significantly reduced in post‐MACs samples (9.61 ± 5.62%) compared with pre‐MACs controls (12.43 ± 6.29%) (P < 0.05). It can be concluded that MACs technique is a simple, noninvasive, technique that can efficiently reduce DNA fragmentation in infertile varicocele patients prior to ART.
Bibliography:ark:/67375/WNG-KBX2WCG4-0
istex:CFB55D19EB56FAF81FDCB3909C8FD9D58248E0CA
ArticleID:AND12343
ObjectType-Article-2
SourceType-Scholarly Journals-1
ObjectType-Feature-1
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ObjectType-Article-1
ObjectType-Feature-2
ISSN:0303-4569
1439-0272
DOI:10.1111/and.12343