Oligonucleotide conjugates based on acyclonucleosides and their use in DNA hybridization assays

Oligonucleotide conjugates based on acyclonucleosides and their use in DNA hybridization assays

Synthesis of two oligonucleotide building blocks based on acyclonucleosides ( 10 , 11 ) which enable the introduction of several nonluminescent and luminescent lanthanide(III) chelates to the oligonucleotide structure is described. They were used in an instrument-assisted DNA synthesis in a standard...

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Bibliographic Details
Published in:Tetrahedron Vol. 58; no. 43; pp. 8771 - 8777
Main Authors: Hakala, Harri, Ollikka, Pia, Degerholm, Jenni, Hovinen, Jari
Format: Journal Article
Language:English
Published: Elsevier Ltd 21-10-2002
Subjects:
DNA synthesis
lanthanide(III)
oligonucleotide
lanthanide(III)
DNA synthesis
oligonucleotide
Online Access:Get full text
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Summary:Synthesis of two oligonucleotide building blocks based on acyclonucleosides ( 10 , 11 ) which enable the introduction of several nonluminescent and luminescent lanthanide(III) chelates to the oligonucleotide structure is described. They were used in an instrument-assisted DNA synthesis in a standard manner. A modified deprotection procedure was used to ensure metal complexation. Also the applicability of these oligonucleotide conjugates to DNA hybridization assays is demonstrated. Graphic
ISSN:0040-4020
1464-5416
DOI:10.1016/S0040-4020(02)01056-6