Alkalimonas mucilaginosa sp. nov. and Alkalimonas cellulosilytica sp. nov. isolated from alkaline Lonar lake, India

Two alkaliphilic, Gram‐stain-negative bacterial strains (MEB004 T and MEB108 T ) were isolated from water samples collected from Lonar lake, India. The phylogenetic analysis of their 16S rRNA gene sequences showed the highest similarity to A. delamerensis DSM 18314 T (98.4%), followed by A. amylolyt...

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Published in:	Antonie van Leeuwenhoek Vol. 117; no. 1; p. 88
Main Authors:	Thite, Sonia, Godbole, Devika, Debnath, Mahima, Bhatt, Agrima, Yadav, Amit, Lodha, Tushar, Joseph, Neetha, Kirdat, Kiran, Boruah, Dibyajyoti, Sharma, Roohani, Joshi, Amaraja
Format:	Journal Article
Language:	English
Published:	Cham Springer International Publishing 01-12-2024 Springer Nature B.V
Subjects:	Amino acids Bacterial Typing Techniques Base Composition Biomedical and Life Sciences Carbohydrates Cellulase Chitinase Coenzyme Q8 Deoxyribonucleic acid DNA DNA, Bacterial - genetics Enzymes Ethanolamine Fatty Acids - analysis Gene sequencing Genes Genome, Bacterial Genomic analysis Genomics Glycerol Hybridization Hydrogen-Ion Concentration India Lakes Lakes - microbiology Life Sciences Lipids Medical Microbiology Microbiology New species Nucleic Acid Hybridization Nucleotides Original Paper Phylogeny Plant Sciences Quinones RNA, Ribosomal, 16S - genetics rRNA 16S Sequence Analysis, DNA Soil Science & Conservation Strains (organisms) Water analysis Water Microbiology Water sampling India Alkaliphilic bacteria Exopolysaccharide Lonar lake pH homeostasis Alkalimonas
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Summary:	Two alkaliphilic, Gram‐stain-negative bacterial strains (MEB004 T and MEB108 T ) were isolated from water samples collected from Lonar lake, India. The phylogenetic analysis of their 16S rRNA gene sequences showed the highest similarity to A. delamerensis DSM 18314 T (98.4%), followed by A. amylolytica DSM 18337 T and A. collagenimarina JCM 14267 T (97.9%). The genome sizes of strains MEB004 T and MEB108 T were determined to be 3,858,702 and 4,029,814 bp, respectively, with genomic DNA G + C contents of 51.4 and 51.9%. Average Nucleotide Identity, DNA–DNA Hybridization and Amino Acid Identity values between strains (MEB004 T and MEB108 T ) and A. amylolytica DSM 18337 T were (82.3 and 85.5), (25.0 and 29.2) and (86.7 and 90.2%). Both novel strains produced industrially important enzymes, such as amylase, lipase, cellulase, caseinase, and chitinase at pH 10 evidenced by the genomic presence of carbohydrate-active enzymes encoding genes. Genomic analyses further identified pH tolerance genes, affirming their adaptation to alkaline Lonar Lake. Dominant fatty acids were Summed feature 8 (C 18:1 ω 7 c and/or C 18:1 ω 6 c ), C 16:0, Summed feature 3, Sum In Feature 2 and C 12:0 3OH. The prevalent polar lipids included phosphatidyl ethanolamine, phosphatidyl glycerol, and diphosphatidyl glycerol. The major respiratory quinone was ubiquinone-8. Based on the polyphasic data, we propose the classification of strains MEB004 T and MEB108 T as novel species within the genus Alkalimonas assigning the names Alkalimonas mucilaginosa sp. nov. and Alkalimonas cellulosilytica sp. nov., respectively. The type strains are MEB004 T (= MCC 5208 T = JCM 35954 T = NCIMB 15460 T ) and MEB108 T (= MCC 5330 T = JCM 35955 T = NCIMB 15461 T ).
Bibliography:	ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 23
ISSN:	0003-6072 1572-9699 1572-9699
DOI:	10.1007/s10482-024-01986-6